Identification of Enterocin NKR-5-3C, a Novel Class IIa Bacteriocin Produced by a Multiple Bacteriocin Producer, Enterococcus faecium NKR-5-3

• Published in: Bioscience, biotechnology, and biochemistry Vol. 76; no. 6; pp. 1245 - 1247
• Main Authors: HIMENO, Kohei, FUJITA, Koji, ZENDO, Takeshi, WILAIPUN, Pongtep, ISHIBASHI, Naoki, MASUDA, Yoshimitsu, YONEYAMA, Fuminori, LEELAWATCHARAMAS, Vichien, NAKAYAMA, Jiro, SONOMOTO, Kenji
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 2012; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Amino Acid Motifs - genetics; Amino Acid Sequence; Bacteria; bacteriocin; Bacteriocins - chemistry; Bacteriocins - genetics; Bacteriocins - pharmacology; Base Sequence; Biological and medical sciences; Disulfides - chemistry; enterocin; Enterococcus faecium; Enterococcus faecium - genetics; Enterococcus faecium - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; lactic acid bacteria; Listeria - drug effects; Listeria - growth & development; Molecular Sequence Data; pediocin; Protein Sorting Signals - genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Analysis, Protein; Streptococcaceae; Enterococcus faecium; Lactic acid bacteria; enterocin; Bacteriocin; Bacteria; Micrococcales; Identification; pediocin
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.120089

The structure of enterocin NKR-5-3C, an anti-listerial bacteriocin produced by a multiple bacteriocin producer, Enterococcus faecium NKR-5-3, was determined. Enterocin NKR-5-3C is a novel class IIa bacteriocin that possesses an YGNGL motif sequence and two disulfide bridges in its structure. It is encoded on gene ent53C together with an 18-amino-acid-residue double glycine leader peptide.