In vitro and in vivo growth alter the population dynamic and properties of a Jeryl Lynn mumps vaccine

• Published in: Vaccine Vol. 33; no. 36; pp. 4586 - 4593
• Main Authors: Connaughton, Sarah M, Wheeler, Jun X, Vitková, Eva, Minor, Philip, Schepelmann, Silke
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 26-08-2015; Elsevier Limited; Elsevier Science
• Subjects: Allergy and Immunology; Animals; Animals, Newborn; Biological properties; Brain research; Cells, Cultured; Chick Embryo; Deep sequencing; Disease Models, Animal; Epithelial Cells; Heterogeneity; Hydrocephalus - pathology; Hydrocephalus - virology; Immunization; Infections; Jeryl Lynn; Kidneys; Mumps; Mumps Vaccine - administration & dosage; Mumps Vaccine - immunology; Mumps virus; Mumps virus - growth & development; Mumps virus - immunology; Mutation; Neurovirulence; Population Dynamics; Rats; Studies; Technology, Pharmaceutical - methods; Vaccines; Viral Load; Viral Plaque Assay; Virulence; Virus Cultivation - methods; Viruses; Czech Republic; Deep sequencing; Vaccines; Mumps virus; Neurovirulence; Jeryl Lynn
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2015.06.109

Highlights • Mumps vaccines contain live attenuated viruses that are manufactured in cell substrates. • The production of the JL-CK vaccine in primary canine kidney cells is atypical. • Genetic changes introduced by different cell types have not been investigated. • JL-CK vaccine contains a unique mix of mumps viruses that have acquired a number of mutations. • Growth in cell or animal substrates dramatically alters the population dynamic of JL-CK.