Probing the Mucoadhesive Interactions Between Porcine Gastric Mucin and Some Water-Soluble Polymers

• Published in: Macromolecular bioscience Vol. 15; no. 11; pp. 1546 - 1553
• Main Authors: Albarkah, Yasser A., Green, Rebecca J., Khutoryanskiy, Vitaliy V.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-11-2015; Wiley Subscription Services, Inc
• Subjects: Acrylics; Agglomeration; Animals; Chemical industry; Dispersions; Gastric Mucins - chemistry; Glycols; Hydrogen bonding; isothermal titration calorimetry; mucoadhesion; Oxides; Polyethylene Glycols - chemistry; Polymers; Polymethacrylic Acids - chemistry; porcine gastric mucin; Solubility; Swine; Titration; Titration calorimetry; Water - chemistry; water-soluble polymers; hydrogen bonding; water-soluble polymers; isothermal titration calorimetry; mucoadhesion; porcine gastric mucin
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201500158

This study investigates the structural features of porcine gastric mucin (PGM) in aqueous dispersions and its interactions with water‐soluble polymers (poly(acrylic acid) (PAA), poly(methacrylic acid) (PMAA), poly(ethylene oxide), and poly(ethylene glycol)) using isothermal titration calorimetry, turbidimetric titration, dynamic light scattering, and transmission electron microscopy. It is established that PAA (450 kDa) and PMAA (100 kDa) exhibit strong specific interactions with PGM causing further aggregation of its particles, while PAA (2 kDa), poly(ethylene oxide) (1 000 kDa), and poly(ethylene glycol) (10 kDa) do not show any detectable effects on mucin. Sonication of mucin dispersions prior to their mixing with PAA (450 kDa) and PMAA (100 kDa) leads to more pronounced intensity of interactions.
The structural features of porcine gastric mucin in aqueous dispersions and its interactions with water‐soluble polymers is shown. Sonication of mucin dispersion results in disaggregation of particles. However, the interaction of mucin with poly(carboxylic acids) via hydrogen bonding causes further aggregation of its particles.