Quantification of Antioxidants by Using Chlorpromazine Hydrochloride: Application of the Method to Food and Medicinal Plant Samples

• Published in: Analytical Sciences Vol. 30; no. 2; pp. 251 - 256
• Main Authors: NAGARAJA, Padmarajaiah, ARADHANA, Narayanan, SUMA, Aandamurthy, SHIVAKUMAR, Anantharaman, CHAMARAJA, Nelligere Arkeshwaraiah
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2014; Springer Nature Singapore
• Subjects: Analytical Chemistry; Antioxidants; Antioxidants - analysis; Antioxidants - chemistry; Antioxidants - isolation & purification; Chemistry; Chlorpromazine - chemistry; chlorpromazine hydrochloride; chromium(VI); Food Analysis - methods; free radical; Free Radicals - chemistry; Oxidation-Reduction; Plants, Medicinal - chemistry; Solvents - chemistry; spectrophotometry; Antioxidants; chromium(VI); spectrophotometry; free radical; chlorpromazine hydrochloride
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.30.251

Chlorpromazine hydrochloride (CPH) (3-(2-chloro-phenothiazine-10-yl)-propyl] dimethylamine hydrochloride) has been the subject of a large number of studies employing a broad spectrum of oxidants, and chosen to examine the course of electron transfer reactions. We report on a method to determine the antioxidant activity of some food and medicinal plants using the oxidation of CPH by chromium(VI) to form a stable CPH radical in the 1:1 orthophosphoric acid–ethyl alcohol (OPA-EtOH) medium. The pink color of the control solution was measured at λmax of 530 nm. Nine standard antioxidants have been studied by this method, along with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The EC50, TEC50, antioxidant efficacy and the stoichiometric values for antioxidants have been evaluated. The radical scavenging activity expressed as EC50 ranged from 9.2 μg/mL in Camellia sinensis to 448.18 μg/mL in Cuminum cyminum. The application of a simple and versatile antioxidant capacity assay for dietary polyphenols and medicinal plant extracts, which are commonly used in Ayurveda opens its relevance in the field of antioxidant analysis.