Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: An international study

• Published in: Annals of neurology Vol. 80; no. 1; pp. 160 - 165
• Main Authors: McGuire, Lynne I., Poleggi, Anna, Poggiolini, Ilaria, Suardi, Silvia, Grznarova, Katarina, Shi, Song, de Vil, Bart, Sarros, Shannon, Satoh, Katsuya, Cheng, Keding, Cramm, Maria, Fairfoul, Graham, Schmitz, Matthias, Zerr, Inga, Cras, Patrick, Equestre, Michele, Tagliavini, Fabrizio, Atarashi, Ryuichiro, Knox, David, Collins, Steven, Haïk, Stéphane, Parchi, Piero, Pocchiari, Maurizio, Green, Alison
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-07-2016; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Subjects: Aged; Aged, 80 and over; Bovine spongiform encephalopathy; Brief Communication; Brief Communications; Case-Control Studies; Clinical medicine; Creutzfeldt-Jakob disease; Creutzfeldt-Jakob Syndrome - cerebrospinal fluid; Creutzfeldt-Jakob Syndrome - diagnosis; Female; Humans; Internationality; Male; Middle Aged; Prions - chemistry; Protein Aggregation, Pathological - cerebrospinal fluid; Recombinant Proteins - chemistry; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 0364-5134; 1531-8249
• DOI: 10.1002/ana.24679

Real‐time quaking‐induced conversion (RT‐QuIC) has been proposed as a sensitive diagnostic test for sporadic Creutzfeldt–Jakob disease; however, before this assay can be introduced into clinical practice, its reliability and reproducibility need to be demonstrated. Two international ring trials were undertaken in which a set of 25 cerebrospinal fluid samples were analyzed by a total of 11 different centers using a range of recombinant prion protein substrates and instrumentation. The results show almost complete concordance between the centers and demonstrate that RT‐QuIC is a suitably reliable and robust technique for clinical practice. Ann Neurol 2016;80:160–165