Design and development of a DNA array for rapid detection and identification of multiple tomato vascular wilt pathogens

• Published in: FEMS microbiology letters Vol. 223; no. 1; pp. 113 - 122
• Main Authors: Lievens, Bart, Brouwer, Margreet, Vanachter, Alfons C.R.C., Lévesque, C.André, Cammue, Bruno P.A., Thomma, Bart P.H.J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 06-06-2003; Blackwell Publishing Ltd; Blackwell
• Subjects: Biological and medical sciences; disease control; DNA; DNA array; DNA, Bacterial - analysis; Fundamental and applied biological sciences. Psychology; Fusarium; Fusarium - genetics; Fusarium - isolation & purification; Fusarium oxysporum f. sp. lycopersici; Fusarium wilt; greenhouses; growers; integrated pest management; Internal transcribed spacer region; irrigation water; Lycopersicon esculentum - microbiology; Microbiology; Miscellaneous; monitoring; Mycology; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Array Sequence Analysis - standards; Oligonucleotides; Plant Diseases - microbiology; plant pathogens; plant tissues; rapid methods; Reproducibility of Results; Reverse dot blot; Sensitivity and Specificity; soil; Solanum lycopersicum var. lycopersicum; Tomato; tomatoes; Vascular wilt; Verticillium; Verticillium - genetics; Verticillium - isolation & purification; Verticillium albo-atrum; Verticillium dahliae; Verticillium wilt; DNA array; Internal transcribed spacer region; Reverse dot blot; Verticillium; Fusarium; Vascular wilt; Tomato; Identification; Fungi; Dicotyledones; Angiospermae; Lycopersicon esculentum; Spermatophyta; Fungi Imperfecti; Solanaceae; Detection; Internal transcribed spacer region: Fusarium; Thallophyta
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(03)00352-5

Fusarium wilt, caused by
Fusarium oxysporum f. sp.
lycopersici, and Verticillium wilt, caused by either
Verticillium albo-atrum or
Verticillium dahliae, are devastating diseases of tomato (
Lycopersicon esculentum) found worldwide. Monitoring is the cornerstone of integrated pest management of any disease. The lack of rapid, accurate, and reliable means by which plant pathogens can be detected and identified is one of the main limitations in integrated disease management. In this paper, we describe the development of a molecular detection system, based on DNA array technology, for rapid and efficient detection of these vascular wilt pathogens. We show the utility of this array for the sensitive detection of these pathogens from complex substrates like soil, plant tissues and irrigation water, and samples that are collected by tomato growers in their greenhouses.