Bifidobacterium species lower serum glucose, increase expressions of insulin signaling proteins, and improve adipokine profile in diabetic mice

• Published in: Biomedical Research Vol. 36; no. 1; pp. 63 - 70
• Main Authors: LE, Thi Kim Chung, HOSAKA, Toshio, NGUYEN, Thanh Trung, KASSU, Afework, DANG, Thi Oanh, TRAN, Hong Ba, PHAM, Tran Phuong, TRAN, Quang Binh, LE, Thi Hong Hao, PHAM, Xuan Da
• Format: Journal Article
• Language: English
• Published: Japan Biomedical Research Press 2015; Japan Science and Technology Agency
• Subjects: Adiponectin - genetics; Adiponectin - metabolism; Administration, Oral; Animals; Bifidobacterium; Bifidobacterium - physiology; Blood Glucose - metabolism; Chemokine CCL2 - genetics; Chemokine CCL2 - metabolism; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - diet therapy; Diabetes Mellitus, Experimental - genetics; Gene Expression Regulation - drug effects; I-kappa B Kinase - genetics; I-kappa B Kinase - metabolism; Insulin - blood; Insulin - genetics; Insulin Receptor Substrate Proteins - genetics; Insulin Receptor Substrate Proteins - metabolism; Interleukin-6 - genetics; Interleukin-6 - metabolism; Male; Mice; Mice, Inbred C57BL; Mitogen-Activated Protein Kinase 1 - genetics; Mitogen-Activated Protein Kinase 1 - metabolism; p38 Mitogen-Activated Protein Kinases - genetics; p38 Mitogen-Activated Protein Kinases - metabolism; Probiotics - pharmacology; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Signal Transduction; Streptozocin
• ISSN: 0388-6107; 1880-313X
• DOI: 10.2220/biomedres.36.63

This study, using C57BL/6J mice with streptozotocin (STZ)-induced diabetes, aimed to determine whether Bifidobacterium species (spp.) both induces the expressions of proteins in the insulin signaling pathway and enhances the expressions of certain adipocytokines. The protein expressions of IκB kinase alpha (IKKα), IκB kinase beta (IKKβ), nuclear factor-kappaB inhibitor alpha (IκBα), and the mitogen-activated protein kinase (MAPK) pathway were also investigated. Oral administration of Bifidobacterium spp. reduced blood glucose levels significantly and increased the protein expressions of insulin receptor beta, insulin receptor substrate 1, protein kinase B (Akt/PKB), IKKα, and IκBα. Extracellular-signal-regulated kinase 2 (ERK2) showed increased expression. Bifidobacterium spp. also induced the adiponectin expression and decreased both macrophage chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) expression. In addition, IKKβ, c-Jun NH2-terminal kinase (JNK) and p38 MAP kinase expressions showed no significant changes in both groups. In conclusion, Bifidobacterium spp. may be the promising bacteria for treating diabetes.