Bacterial microleakage in endodontically treated teeth following two methods of postspace preparation at two-time intervals: An in vitro study

• Published in: The journal of Indian Prosthodontic Society Vol. 22; no. 3; pp. 233 - 239
• Main Authors: Mostafavi, Azam, Rasoulzadehsheikh, Mahsa, Meraji, Naghmeh, Pourhajibagher, Maryam
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01-07-2022; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: Analysis; Bacteria; dental instruments; dental leakage; Dental Leakage - microbiology; Endodontics; Ethylenediaminetetraacetic acid; Gutta-Percha; Heat; Humans; Methods; Microleakage; Post and Core Technique; Root Canal Filling Materials; Root canal therapy; Root canals; Statistical analysis; Survival; Teeth; time; Tooth, Nonvital - therapy; heat; dental leakage; time; Dental instruments; post and core technique
• ISSN: 0972-4052; 1998-4057
• DOI: 10.4103/jips.jips_441_21

Aims: The goal of this study was to analyze the bacterial microleakage following two methods (heat vs. rotary) of postspace preparation after two-time intervals (immediate vs. 1 week later).
Setting and Design: In vitro-comparative study.
Materials and Methods: Eighty-two single-rooted teeth were decoronated at the cementoenamel junction. Root canals were prepared using rotary files. After root canal obturation, specimens were randomly allocated to 4 experimental groups based on the method of postspace preparation (heat or peeso reamer) and time interval (immediate or 1 week later) (n = 18). Group 1: Peeso reamer-immediate, Group 2: Heat-immediate, Group 3: Peeso reamer-1 week later, Group 4: Heat-1 week later. 10 specimens were considered as positive and negative controls (n = 5 each). Custom-made dual-chamber devices were used to appraise the bacterial microleakage for 60 days.
Statistical Analysis Used: Data were analyzed with Chi-Square and Log-Rank tests and Cox regression.
Results: All through the experimental period, there was no significant difference (P = 0.41) between the studied groups. Groups 2 and 4 had the highest microleakage and the lowest survival rate (55.56% ± 11.71%). Group 1 showed the lowest microleakage and the highest survival rate (77.8% ± 9.80%).
Conclusions: The applied techniques for postspace preparation and the time intervals (neither independently nor simultaneously) showed no significant difference in the field of bacterial leakage.