An in Vitro Neurite-Promoting Antigen Functions in Axonal Regeneration in vivo

• Published in: Science (American Association for the Advancement of Science) Vol. 237; no. 4822; pp. 1605 - 1608
• Main Authors: Sandrock, Alfred W., Matthew, William D.
• Format: Journal Article
• Language: English
• Published: Washington, DC The American Association for the Advancement of Science 25-09-1987; American Association for the Advancement of Science
• Subjects: Animals; Anterior chamber; Antibodies; Antibodies, Monoclonal; Antigens; Antigens - physiology; Axons; Axons - physiology; Biochemistry; Biological and medical sciences; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Heparin - analogs & derivatives; Heparin - immunology; Hybridomas; Hybridomas - transplantation; Innervation; Iris; Iris - innervation; Laminin - physiology; Nerve fibers; Nerve Regeneration; Nervous system; Neurites; Norepinephrine; Norepinephrine - metabolism; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Proteoglycans - immunology; Rats; Regeneration; Sympathetic Nervous System - metabolism; Sympathetic Nervous System - physiology; Vertebrates: nervous system and sense organs; Iridaceae; Monocotyledones; Rat; Rodentia; Antigen; Iris; Regeneration; Vertebrata; Mammalia; Angiospermae; Spermatophyta; Norepinephrine; Sympathic nervous system
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.3306923

The function of the neurite growth-promoting antigen INO has been tested in an in vivo neurite regeneration system, the rat iris. The sympathetic innervation of the irides was removed by a single systemic injection of 6-hydroxydopamine. The subsequent regeneration of sympathetic axons into the iris of one eye bathed by the INO antibody, which inhibits neurite growth in vitro, was compared with the regrowth of sympathetic axons into the iris of the animal's other eye, which contained control antibody. Antibodies were released within the eye by implanted hybridoma cells. Neurite regeneration was measured by assaying [$^{3}$H]norepinephrine uptake into freshly explanted irides. The blockage of the function of the INO antigen by the antibody resulted in a decreased rate of axonal regeneration, thus suggesting the involvement of the INO antigen in the process of neurite regeneration in vivo.