Characterization of human peritoneal monocyte/macrophage subsets in homeostasis: Phenotype, GATA6, phagocytic/oxidative activities and cytokines expression

• Published in: Scientific reports Vol. 8; no. 1; pp. 12794 - 14
• Main Authors: Ruiz-Alcaraz, Antonio José, Carmona-Martínez, Violeta, Tristán-Manzano, María, Machado-Linde, Francisco, Sánchez-Ferrer, María Luisa, García-Peñarrubia, Pilar, Martínez-Esparza, María
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24-08-2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/1; 13/31; 631/250/24/2510; 631/250/2504/342/1956; 692/308/53/2421; 692/420/256/2515; 692/699/249/2510; CD11b antigen; CD11c antigen; CD14 antigen; CD16 antigen; CD40 antigen; CD80 antigen; CD86 antigen; Cytokines; Flow cytometry; Genotype & phenotype; Histamine; Histocompatibility antigen HLA; Homeostasis; Humanities and Social Sciences; Inflammatory diseases; Interleukin 10; Interleukin 6; Intracellular; Ion exchange; L-selectin; Macrophages; Monocytes; multidisciplinary; Peritoneum; Phagocytes; Phenotypes; Science; Science (multidisciplinary); Tumor necrosis factor-α
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-30787-x

Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on the characteristics of human peritoneal macrophages in homeostasis. Here, we have studied by flow cytometry several features of human peritoneal macrophages obtained from the peritoneal cavity of healthy women. Three peritoneal monocyte/macrophage subsets were established on the basis of CD14/CD16 expression (CD14
++
CD16
−
, CD14
++
CD16
+
and CD14
high
CD16
high
), and analysis of CD11b, CD11c, CD40, CD62L, CD64, CD80, CD86, CD116, CD119, CD206, HLA-DR and Slan was carried out in each subpopulation. Intracellular expression of GATA6 and cytokines (pro-inflammatory IL-6 and TNF-α, anti-inflammatory IL-10) as well as their phagocytic/oxidative activities were also analyzed, in an attempt to identify genuine resident peritoneal macrophages. Results showed that human peritoneal macrophages are heterogeneous regarding their phenotype, cell complexity and functional abilities. A direct relationship of CD14/CD16 expression, intracellular content of GATA6, and activation/maturation markers like CD206 and HLA-DR, support that the CD14
high
CD16
high
subset represents the mature phenotype of steady-state human resident peritoneal macrophages. Furthermore, increased expression of CD14/CD16 is also related to the phagocytic activity.