A rapid agglutination assay for canine brucellosis using antigen coated beads

• Published in: Journal of Veterinary Medical Science Vol. 69; no. 5; pp. 477 - 480
• Main Authors: Watarai, M.(Obihiro Univ. of Agriculture and Veterinary Medicine, Hokkaido (Japan)), Kim, S, Yamamoto, J, Miyahara, K, Kazama, M, Matsuoka, S, Chimura, S, Suzuki, H
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-05-2007; Japan Science and Technology Agency
• Subjects: agglutination test; AGGLUTINATION TESTS; Agglutination Tests - methods; Agglutination Tests - veterinary; Animals; Antigens; ATP-Binding Cassette Transporters - analysis; Brucella; Brucella canis; Brucella canis - immunology; BRUCELLOSE; BRUCELLOSIS; Brucellosis - diagnosis; Brucellosis - veterinary; BRUCELOSIS; canine; Canis; CHIEN; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; Dog Diseases - diagnosis; DOGS; LATEX; latex beads; METHODE; METHODS; METODOS; Microspheres; PERRO; REACCIONES DE AGLUTINACION; REACTION D'AGGLUTINATION; Superoxide Dismutase - analysis
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.69.477

Brucella canis is the causative agent of canine brucellosis and facultative intracellular pathogen. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. In this study, crude antigens were extracted from B. canis using hot saline, coated on to latex beads and their usefulness in the serological diagnosis of canine brucellosis was examined. Mixing the antigen coated latex beads with the sera of dogs infected with B. canis produced clear agglutination, but this was not so for B. canis free dog sera. N-terminal amino acid sequence analysis of the crude hot saline extracts showed that they contained copper-zinc superoxide dismutase, ribose ABC transporter and hypothetical protein of Brucella as antigens. A serological survey of canine serum samples conducted by means of an agglutination test using the antigen coated latex beads, showed that this method was more specific than the tube agglutination test using whole bacterial cell antigens. Although these results suggest that our method in which crude hot saline extracted antigens are coated on to latex beads would be useful in the serological diagnosis of canine brucellosis, we need further investigation using more serum samples to confirm the usefulness of our method.