Tumor-associated macrophage subtypes on cancer immunity along with prognostic analysis and SPP1-mediated interactions between tumor cells and macrophages

• Published in: PLoS genetics Vol. 20; no. 4; p. e1011235
• Main Authors: Xu, Liu, Chen, Yibing, Liu, Lingling, Hu, Xinyu, He, Chengsi, Zhou, Yuan, Ding, Xinyi, Luo, Minhua, Yan, Jiajing, Liu, Quentin, Li, Hongsheng, Lai, Dongming, Zou, Zhengzhi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22-04-2024; Public Library of Science (PLoS)
• Subjects: AKT protein; Antigens; Apoptosis; beta Catenin - genetics; beta Catenin - metabolism; Biology and Life Sciences; Breast cancer; Cancer; Cancer therapies; Cell activation; Cell Communication - immunology; Cell cycle; Cell differentiation; Cell interactions; Cell Line, Tumor; Cell research; Cytokines; Epithelial cells; Gene expression; Gene Expression Regulation, Neoplastic; Genotype & phenotype; Humans; Immunity; Immunotherapy; Liver cancer; Lung cancer; Lymphocytes T; Macrophages; Macrophages - immunology; Macrophages - metabolism; Medicine and Health Sciences; Melanoma; N-Cadherin; Neoplasms - genetics; Neoplasms - immunology; Osteopontin - genetics; Osteopontin - metabolism; PD-1 protein; Physiological aspects; Prognosis; Signal Transduction; Single-Cell Analysis; Stat3 protein; STAT3 Transcription Factor - genetics; STAT3 Transcription Factor - metabolism; Tumor antigens; Tumor cells; Tumor-Associated Macrophages - immunology; Tumor-Associated Macrophages - metabolism; Tumors; Viral infections; β-Catenin; China; Henan China
• ISSN: 1553-7404; 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1011235

Tumor-associated macrophages (TAM) subtypes have been shown to impact cancer prognosis and resistance to immunotherapy. However, there is still a lack of systematic investigation into their molecular characteristics and clinical relevance in different cancer types. Single-cell RNA sequencing data from three different tumor types were used to cluster and type macrophages. Functional analysis and communication of TAM subpopulations were performed by Gene Ontology-Biological Process and CellChat respectively. Differential expression of characteristic genes in subpopulations was calculated using zscore as well as edgeR and Wilcoxon rank sum tests, and subsequently gene enrichment analysis of characteristic genes and anti-PD-1 resistance was performed by the REACTOME database. We revealed the heterogeneity of TAM, and identified eleven subtypes and their impact on prognosis. These subtypes expressed different molecular functions respectively, such as being involved in T cell activation, apoptosis and differentiation, or regulating viral bioprocesses or responses to viruses. The SPP1 pathway was identified as a critical mediator of communication between TAM subpopulations, as well as between TAM and epithelial cells. Macrophages with high expression of SPP1 resulted in poorer survival. By in vitro study, we showed SPP1 mediated the interactions between TAM clusters and between TAM and tumor cells. SPP1 promoted the tumor-promoting ability of TAM, and increased PDL1 expression and stemness of tumor cells. Inhibition of SPP1 attenuated N-cadherin and β-catenin expression and the activation of AKT and STAT3 pathway in tumor cells. Additionally, we found that several subpopulations could decrease the sensitivity of anti-PD-1 therapy in melanoma. SPP1 signal was a critical pathway of communication between macrophage subtypes. Some specific macrophage subtypes were associated with immunotherapy resistance and prognosis in some cancer types.