Molecular analysis of the endobronchial stent microbial biofilm reveals bacterial communities that associate with stent material and frequent fungal constituents

• Published in: PloS one Vol. 14; no. 5; p. e0217306
• Main Authors: McGinniss, John E, Imai, Ize, Simon-Soro, Aurea, Brown, Melanie C, Knecht, Vincent R, Frye, Laura, Ravindran, Priyanka M, Dothard, Marisol I, Wadell, Dylan A, Sohn, Michael B, Li, Hongzhe, Christie, Jason D, Diamond, Joshua M, Haas, Andrew R, Lanfranco, Anthony R, DiBardino, David M, Bushman, Frederic D, Collman, Ronald G
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29-05-2019; Public Library of Science (PLoS)
• Subjects: Airway obstruction; Bacteria - genetics; Biofilms - growth & development; Biology and Life Sciences; Bronchi - microbiology; Bronchi - surgery; Care and treatment; DNA sequencing; Female; Fungi; Fungi - genetics; Genes; Humans; Male; Materials; Medicine and Health Sciences; Microbial colonies; Microbial mats; Microbiota; Organ transplantation; Pathogenic microorganisms; Physical Sciences; Research and Analysis Methods; RNA; RNA, Ribosomal, 16S - genetics; Silicones; Stents; Stents - adverse effects; Stents - microbiology; Surgery; Technology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0217306

Endobronchial stents are increasingly used to treat airway complications in multiple conditions including lung transplantation but little is known about the biofilms that form on these devices. We applied deep sequencing to profile luminal biofilms of 46 endobronchial stents removed from 20 subjects primarily with lung transplantation-associated airway compromise. Microbial communities were analyzed by bacterial 16S rRNA and fungal ITS marker gene sequencing. Corynebacterium was the most common bacterial taxa across biofilm communities. Clustering analysis revealed three bacterial biofilm types: one low diversity and dominated by Corynebacterium; another was polymicrobial and characterized by Staphylococcus; and the third was polymicrobial and associated with Pseudomonas, Streptococcus, and Prevotella. Biofilm type was significantly correlated with stent material: covered metal with the Staphylococcus-type biofilm, silicone with the Corynebacterium-dominated biofilm, and uncovered metal with the polymicrobial biofilm. Subjects with sequential stents had frequent transitions between community types. Fungal analysis found Candida was most prevalent, Aspergillus was common and highly enriched in two of three stents associated with airway anastomotic dehiscence, and fungal taxa not typically considered pathogens were highly enriched in some stents. Thus, molecular analysis revealed a complex and dynamic endobronchial stent biofilm with three bacterial types that associate with stent material, a central role for Corynebacterium, and that both expected and unexpected fungi inhabit this unique niche. The current work provides a foundation for studies to investigate the relationship between stent biofilm composition and clinical outcomes, mechanisms of biofilm establishment, and strategies for improved stent technology and use in airway compromise.