Ciprofloxacin-resistant, CTX-M-15-producing Escherichia coli ST131 clone in extraintestinal infections in Italy

• Published in: Clinical microbiology and infection Vol. 16; no. 10; pp. 1555 - 1558
• Main Authors: Cerquetti, M., Giufrè, M., García-Fernández, A., Accogli, M., Fortini, D., Luzzi, I., Carattoli, A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01-10-2010; Blackwell Publishing Ltd; Wiley-Blackwell; Elsevier Limited
• Subjects: Amino Acid Substitution - genetics; Anti-Bacterial Agents - pharmacology; Bacterial diseases; Bacterial diseases of the urinary system; Bacterial sepsis; Bacterial Typing Techniques; beta -Lactam antibiotics; beta-Lactamases - biosynthesis; Biological and medical sciences; Cephalosporins; Cholera; Ciprofloxacin; Ciprofloxacin - pharmacology; Cluster Analysis; DNA Topoisomerase IV - genetics; Drug Resistance, Multiple, Bacterial; E coli; Escherichia coli; Escherichia coli - classification; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - isolation & purification; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Fluoroquinolones; Human bacterial diseases; Humans; IncF plasmids; Infection; Infectious diseases; Medical sciences; Microbial Sensitivity Tests; Molecular Epidemiology; Molecular Typing; multidrug resistance; Mutation, Missense; Nephrology. Urinary tract diseases; replicon typing; Rome - epidemiology; septicemia; ST131; urinary infections; Urinary system involvement in other diseases. Miscellaneous; Urinary tract. Prostate gland; Italy; Europe; Rome; multidrug resistance; IncF plasmids; septicemia; replicon typing; ST131; urinary infections; Urinary system disease; Typing; Septicemia; Escherichia coli; Urinary tract disease; Plasmid; Urinary tract infection; Microbiological investigation; Multiple resistance; Bacteria; Enterobacteriaceae
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2010.03162.x

Quinolone and ß-lactam resistance mechanisms and clonal relationships were characterized among Escherichia coli isolates resistant to ciprofloxacin and extended-spectrum cephalosporins associated with human extra-intestinal infections in Rome. The E. coli. ST131 clone was found to be prevalent. This clone invariably carried a specific pattern of substitutions in the topoisomerase genes and all isolates but one produced CTX-M-15. One ST131 isolate produced SHV-12. The new ST131 variant described here is of particular concern because it combines fluoroquinolone resistance and chromosomally encoded CTX-M-15.