Tocotrienol-Rich Fraction Prevents Cell Cycle Arrest and Elongates Telomere Length in Senescent Human Diploid Fibroblasts

• Published in: Journal of biomedicine & biotechnology Vol. 2011; no. 2011; pp. 1 - 11
• Main Authors: Makpol, Suzana, Durani, Lina Wati, Kien Hui, Chua, Mohd Yusof, Yasmin Anum, Wan Ngah, Wan Zurinah
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01-01-2011; Hindawi Publishing Corporation; Dar al -Nasr -al-Llktruni; John Wiley & Sons, Inc
• Subjects: beta-Galactosidase - metabolism; Biological and medical sciences; Cell cycle; Cell Cycle - drug effects; Cell Shape - drug effects; Cells, Cultured; Cellular Senescence - drug effects; Chemical Fractionation; Comet Assay; Diploidy; DNA Damage; Fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - enzymology; Fibroblasts - metabolism; Health aspects; Humans; Medical sciences; Pharmacology. Drug treatments; Physiological aspects; Staining and Labeling; Telomerase - metabolism; Telomere - metabolism; Telomeres; Tocotrienols; Tocotrienols - pharmacology; Malaysia; Human; Prevention; Diploidy; Cell fraction; Length; Cell cycle; Fibroblast; Telomere
• ISSN: 1110-7243; 1110-7251
• DOI: 10.1155/2011/506171

This study determined the molecular mechanisms of tocotrienol-rich fraction (TRF) in preventing cellular senescence of human diploid fibroblasts (HDFs). Primary culture of HDFs at various passages were incubated with 0.5 mg/mL TRF for 24 h. Telomere shortening with decreased telomerase activity was observed in senescent HDFs while the levels of damaged DNA and number of cells in G0/G1 phase were increased and S phase cells were decreased. Incubation with TRF reversed the morphology of senescent HDFs to resemble that of young cells with decreased activity of SA-β-gal, damaged DNA, and cells in G0/G1 phase while cells in the S phase were increased. Elongated telomere length and restoration of telomerase activity were observed in TRF-treated senescent HDFs. These findings confirmed the ability of tocotrienol-rich fraction in preventing HDFs cellular ageing by restoring telomere length and telomerase activity, reducing damaged DNA, and reversing cell cycle arrest associated with senescence.