Increased mRNA expression of key cytokines among suspected cases of Pneumocystis jirovecii infection

• Published in: BMC infectious diseases Vol. 21; no. 1; p. 28
• Main Authors: Alshahrani, Mohammad Y, Alfaifi, Mohammed, Al Shahrani, Mesfer, Alshahrani, Abdulaziz S, Alkhathami, Ali G, Dera, Ayed A, Ahmad, Irfan, Wahab, Shadma, Beg, Mirza M A, Hakamy, Ali, Hamid, Mohamed E
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07-01-2021; BioMed Central; BMC
• Subjects: Adult; Age groups; Azure Stains; Case-Control Studies; Chest; Cross-Sectional Studies; Cytokines; Cytokines - blood; Cytokines - genetics; Cytotoxicity; Diagnosis; Dyspnea; Female; Fever; Fluorescence; Fluorescent Antibody Technique; Gene Expression; Health aspects; Hospitals; Humans; Immune-compromised; Immune-fluorescent staining; Immunology; Infections; Infectious diseases; Inflammation; Interleukin 10; Interleukin 13; Interleukin 2; Interleukin 4; Interleukin-10 - genetics; Interleukin-13 - genetics; Interleukin-2 - genetics; Interleukin-4 - genetics; Interleukins (ILs); Lymphadenopathy; Male; Messenger RNA; Middle Aged; Morbidity; Patients; PCR; Pleural effusion; Pneumocystis carinii - genetics; Pneumocystis carinii - pathogenicity; Pneumocystis carinii pneumonia; Pneumocystis jirovecii; Pneumonia, Pneumocystis - diagnostic imaging; Pneumonia, Pneumocystis - genetics; Pneumonia, Pneumocystis - microbiology; Pneumothorax; Polymerase Chain Reaction; Respiration; Risk factors; RNA, Messenger - blood; Saudi Arabia; Staining; Statistical analysis; Tolonium Chloride; Toluidine; Toluidine blue; Weight loss; Wheezing; X-rays; Saudi Arabia; Saudi Arabia; Immune-compromised; Interleukins (ILs); Immune-fluorescent staining; PCR
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-020-05729-6

Pneumocystis pneumonia (PCP) is a fatal infectious disease caused by Pneumocystis jirovecii (PJP). The major factor relevant to morbidity and mortality seems to be the host inflammatory reaction. The objective of this study was to evaluate the role of IL-2, IL-4, IL-10, and IL-13 cytokine mRNA expression among suspected P. jirovecii infection.
This was a cross-sectional analytical study undertaken in Aseer region, Saudi Arabia. One hundred suspected PCP cases and 100 healthy controls were included in the study. Basic clinical manifestations, radiological findings, microbiological and immunological findings were extracted from the hospital records from January 2019 to August 2019, Pneumocystis detection was done by immune-fluorescent staining (IFAT, Gomorimethanamine silver staining (GMSS), Giemsa staining, Toluidine blue O (TBO), and Pneumocystis RT-PCR.
Increased more than 5 fold, 3 fold, 4 fold, and 7 fold of IL-2, IL-4, IL-10, and IL-13 mRNA expression were observed in PCP cases compared to controls. Higher expression of IL-2 mRNA was connected with crept, wheezing and chest X-ray findings like central perihilar infiltrate, patchy infiltrate, consolidation, hilar lymphadenopathy, pneumothorax, pleural effusion which showed higher expression compared to counterpart (p< 0.0001). Higher expression of IL-4 mRNA was found to be significantly associated with weight loss (p=0.002), dyspnea (p=0.003), crept (p=0.01), and chest X-ray findings (p< 0.0001). Significantly increased expression of IL-10 mRNA was observed to be associated with weight loss, dyspnea, night sweats, wheezing, and different findings of chest X-ray compared to their counterparts, whereas, IL-13 mRNA was observed in cases with fever. Suspected cases of PCP confirmed positive by IFTA with higher IL-2, IL-4, and IL-10 mRNA expression compared to negative cases. RT-PCR confirmed PCP cases had significantly higher expression of IL-2, IL-4, and IL-10 as well as IL-13 mRNA compared to negative cases. Positive detected cases by GMSS showed higher IL-2, IL-10 mRNA expression, while Giemsa showed only higher IL-4 mRNA expression compared to negative cases.
Confirmed cases of P. jirovecii showed higher IL-2, IL-4, IL-10, and IL-13 mRNA expression comparatively to negative cases. Increased expression of cytokines may be indicative of infection severity and could help in patients' management.