Regulation of14C-cholesterol uptake from mixed micelles by organ culture of the mucosa epithelium from human small intestine: Effects of selective ACAT inhibitor

• Published in: Bulletin of experimental biology and medicine Vol. 123; no. 2; pp. 139 - 143
• Main Authors: Repin, V. S., Bocharov, A. V., Vishnyakova, T. G., Baranova, I. N., Kornelius, L., Kornyushin, M. A., Talalaev, A. G., Kask, L. N., Mironyuk, O. V., Pokrovskii, A. V., Zotikov, A. E.
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-02-1997
• Subjects: Cholesterol; Epithelium; Esters; Micelles; Organ culture; Proteins; Small intestine
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/BF02766435

The initial rate of14C-cholesterol uptake from mixed micelles is studied using organ culture of mucosal epithelium from human small intestine obtained during surgery. Intense, reproducible, and ACAT-regulated level of specific14C-cholesterol uptake is monitored during the first 3 h of culturing. The specific14C-cholesterol uptake is assessed by the difference between the total uptake of14C-cholesterol and nonspecific uptake of14C-sitosterol from the micelles. It is shown that less than 20% of absorbed14C-cholesterol is converted into cholesterol esters. C44788, a high-affinity and selective ACAT inhibitor, inhibits14C-cholesterol uptake in a dose-dependent manner and practically has no effect on14C-oleate uptake.