Pathological characteristics of Ccdc85c knockout rats: a rat model of genetic hydrocephalus

• Published in: Experimental Animals Vol. 69; no. 1; pp. 26 - 33
• Main Authors: Konishi, Shizuka, Tanaka, Natsuki, Mashimo, Tomoji, Yamamoto, Takashi, Sakuma, Tetsushi, Kaneko, Takehito, Tanaka, Miyuu, Izawa, Takeshi, Yamate, Jyoji, Kuwamura, Mitsuru
• Format: Journal Article
• Language: English
• Published: Japan Japanese Association for Laboratory Animal Science 01-01-2020; Japan Science and Technology Agency
• Subjects: Animals; Ccdc85c; Cerebrum; Disease Models, Animal; Gene deletion; Hemorrhage; Hydrocephalus; Hydrocephalus - genetics; Hydrocephalus - pathology; Mice; Mutation; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Nuclease; Original; Phenotype; Phenotypes; rat; Rats; Rodents; subcortical heterotopia; Transcription; Transcription activator-like effector nucleases; subcortical heterotopia; hydrocephalus; rat; Ccdc85c
• ISSN: 1341-1357; 1881-7122
• DOI: 10.1538/expanim.19-0005

Spontaneous hhy mice show hydrocephalus and subcortical heterotopia, and a mutation in the Ccdc85c gene has been identified. To contribute to the comparison of the role of Ccdc85c in different species, we established a Ccdc85c KO rat and investigated its pathological phenotypes. Ccdc85c KO rats were produced by genomic engineering using transcription activator-like effector nuclease (TALEN). The KO rats had an approximately 350-bp deletion in Ccdc85c and lacked CCDC85C protein expression. The KO rats showed non-obstructive hydrocephalus, subcortical heterotopia, and intracranial hemorrhage. The KO rats had many pathological characteristics similar to those in hhy mice. These results indicate that CCDC85C plays an important role in cerebral development in rats, and the function of CCDC85C in the cerebrum are similar in rats and mice.