Biocompatibility of three bioabsorbable membranes assessed in FGH fibroblasts and human osteoblast like cells culture

• Published in: Head & face medicine Vol. 10; no. 1; p. 29
• Main Authors: Soares, Michelle Pereira Costa Mundim, Soares, Paulo Vinícius, Pereira, Analice Giovani, Moura, Camilla Christian Gomes, Soares, Priscila Barbosa Ferreira, Naves, Lucas Zago, de Magalhães, Denildo
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 06-08-2014; BioMed Central
• Subjects: Absorbable Implants; Analysis; Biocompatibility; Biocompatible Materials; Biomedical materials; Bone morphogenetic proteins; Cell Culture Techniques; Cell Proliferation; Collagen; Colleges & universities; Dentistry; Fibroblasts - ultrastructure; Gingiva - cytology; Graduate students; Guided Tissue Regeneration, Periodontal - methods; Humans; Ligaments; Materials Testing; Membranes; Membranes, Artificial; Microscopy, Electron, Scanning; Osteoblasts - ultrastructure; Physiological aspects; Polylactic acid; Reproducibility of Results; Spectrophotometry; Tissue engineering
• ISSN: 1746-160X; 1746-160X
• DOI: 10.1186/1746-160X-10-29

Specific physical and chemical features of the membranes may influence the healing of periodontal tissues after guided tissue regeneration (GTR). The aim of the present investigation was to analyze the biological effects of three bioabsorbable membranes. The hypothesis is that all tested membranes present similar biological effects.
Human osteoblast like-cells (SaOs-2) and gingival fibroblasts FGH (BCRJ -RJ) were cultured in DMEM medium. The viability of the cells cultured on the membranes was assesses using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Quantitative determination of activated human Transforming Growth Factor beta 1 (TGF-β1) on the supernatants of the cell culture was observed. Samples were examined using scanning electron microscope (SEM).
SaOs2, in 24 hours, PLA group showed higher values when compared to other groups (P < 0.05). All groups presented statistical significance values when compared two times. In 4 h and 24 h, for the fibroblasts group, significantly difference was found to PLA membrane, when compared with the other groups (p < 0.05). For TGFβ1 analyzes, comparing 4 and 24 h, for the osteoblast supernatant, COL1 and PLA groups showed statistically significant difference (p <0,008). On the analysis of culture supernatants of fibroblasts, in 24 hours, only PLA group presented significant difference (p = 0,008).
The biomaterials analyzed did not show cytotoxicity, since no membrane presented lower results than the control group. PLA membrane presented the best performance due to its higher cell viability and absorbance levels of proliferation. Both collagen membranes showed similar results either when compared to each other or to the control group.