First insights into the genetic characteristics and drug resistance of Mycobacterium tuberculosis population collected during the first national tuberculosis prevalence survey of Lao PDR (2010-2011)

• Published in: BMC infectious diseases Vol. 19; no. 1; p. 851
• Main Authors: Somphavong, Silaphet, Berland, Jean-Luc, Gauthier, Marie, Vu, Thi Thuong, Nguyen, Quang Huy, Iem, Vibol, Vongvichit, Phouvang, Inthavong, Donekham, Akkhavong, Vanthala, Chanthavilay, Phetsavanh, Soundala, Sengaloun, Keovichit, Inthalaphone, Paranhos-Baccalà, Glaucia, Paboriboune, Phimpha, Nguyen, Thi Van Anh, Bañuls, Anne-Laure
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15-10-2019; BioMed Central; BMC
• Subjects: Adolescent; Adult; Antitubercular Agents - pharmacology; Antitubercular Agents - therapeutic use; Cluster Analysis; Clustering; Control; Deoxyribonucleic acid; Disease transmission; DNA; Drug resistance; Drug resistance in microorganisms; Drug Resistance, Multiple, Bacterial - drug effects; Drug-resistant tuberculosis; Epidemiology; Female; Genetic testing; Genotype; Genotypes; Health aspects; Humans; Infectious diseases; Isoniazid; Isoniazid - pharmacology; Isoniazid - therapeutic use; Lao PDR; Laos - epidemiology; Male; Middle Aged; Molecular epidemiology; Mycobacterium tuberculosis; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Mycobacterium tuberculosis family; Patient outcomes; Phylogeny; Polls & surveys; Population; Prevalence; Prevention; Rifampin; Rifampin - pharmacology; Rifampin - therapeutic use; Risk factors; Spoligotyping; Studies; Surveillance systems; Surveys; Tuberculosis; Tuberculosis, Multidrug-Resistant - diagnosis; Tuberculosis, Multidrug-Resistant - drug therapy; Tuberculosis, Multidrug-Resistant - epidemiology; Young Adult; Laos; Beijing China; Vietnam; China; India; Drug-resistant tuberculosis; Molecular epidemiology; Lao PDR; Mycobacterium tuberculosis family
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-019-4435-z

In Lao People's Democratic Republic (PDR), tuberculosis (TB) prevalence was estimated at 540/100,000 in 2011. Nevertheless, little is known about the genetic characteristics and anti-TB drug resistance of the Mycobacterium tuberculosis population. The main objective of this work was to study the genetic characteristics and drug resistance of M. tuberculosis population collected during the first National TB Prevalence Survey (TBPS) of Lao PDR (2010-2011).
Two hundred and twenty two isolates collected during TBPS (2010-2011) were analyzed with the GenoType MTBDRplus test for M. tuberculosis identification and drug resistance detection. Then, 206 of the 222 isolates were characterized by spoligotyping and MIRU-VNTR typing.
Among the 222 M. tuberculosis isolates, 11 were mono-resistant to isoniazid and 2 were resistant to isoniazid and rifampicin (MDR-TB), using the GenoType MTBDRplus test. Among the 202 genetically characterized isolates, the East African-Indian (EAI) family was predominant (76.7%) followed by the Beijing (14.4%) and T (5.5%) families. EAI isolates came from all the country provinces, whereas Beijing isolates were found mainly in the northern and central provinces. A higher proportion of Beijing isolates was observed in people younger than 35 years compared to EAI. Moreover, the percentage of drug resistance was higher among Beijing (17.2%) than EAI (5.2%) isolates, and the two MDR-TB isolates belonged to the Beijing family. Combined analysis of the MIRU-VNTR and spoligotyping results (n = 202 isolates) revealed an estimated clustering rate of 11% and the occurrence of mini-outbreaks of drug-resistant TB caused by Beijing genotypes.
The EAI family, the ancient and endemic family in Asia, is predominant in Lao PDR whereas the prevalence of Beijing, the most harmful M. tuberculosis family for humans, is still low, differently from neighboring countries. However, its association with drug resistance, its presence in young patients and its potential association with recent transmission suggest that the Beijing family could change TB epidemiological pattern in Lao PDR. Therefore, efficient TB control and surveillance systems must be maintained and reinforced to prevent the emergence of highly transmissible and drug-resistant strains in Lao PDR, as observed in neighboring countries.