Metal-organic framework-based nanoplatform enhance fibroblast activity to treat periodontitis

• Published in: Dental Materials Journal Vol. 42; no. 1; pp. 19 - 29
• Main Authors: JIAO, Jian, TIAN, Yujuan, LI, Ying, LIANG, Yunkai, DENG, Shu, WANG, Wanmeng, WANG, Yuwei, LIN, Yi, TIAN, Yuan, LI, Changyi
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society for Dental Materials and Devices 25-01-2023; Japanese Society for Dental Materials and Devices; Japan Science and Technology Agency
• Subjects: Accelerated tests; Animals; Baicalein; Cell adhesion; Cell migration; Cell proliferation; Collagen; Fibroblasts; Gum disease; Immunofluorescence; Inflammation; Mesoporous Prussian blue; Metal-organic frameworks; Metal-Organic Frameworks - pharmacology; Nanoparticles; Nanoplatform; Periodontitis; Periodontitis - drug therapy; Periodontium; Pigments; Rats; Soft tissues; Staining; Vinculin; Vinculin - pharmacology; Wound Healing; Baicalein; Fibroblasts; Mesoporous Prussian blue; Periodontitis; Nanoplatform
• ISSN: 0287-4547; 1881-1361
• DOI: 10.4012/dmj.2022-096

After periodontal tissue injury, reconstruct soft tissue sealing around the tooth surface is of fundamental importance to treat periodontitis. Among multiple cell types, fibroblast plays a central role in reestablishing functional periodontium. To enhance fibroblast activity, a novel metal-organic framework-based nanoplatform is fabricated using mesoporous Prussian blue (MPB) nanoparticles to load baicalein (BA), named MPB-BA. Drug release test displayed sustained BA release of MPB-BA. Cell proliferation, transwell migration and wound healing tests revealed accelerated fibroblast proliferation and migration for the established MPB-BA nanoplatform. Moreover, vinculin immunofluorescence staining, western blot and quantitative real-time PCR analysis showed up-regulated vinculin protein and integrin α5 and integrin β1 gene expressions for MPB-BA, suggesting improved cell adhesion. In addition, hematoxylin and eosin (H&E) and Masson trichromatic staining suggested superior anti-inflammatory and collagen fiber reconstruction effects for MPB-BA in a rat experimental periodontitis model in vivo. Our study may provide a promising strategy for the treatment of periodontitis.