Chlorophytum borivilianum Polysaccharide Fraction Provokes the Immune Function and Disease Resistance of Labeo rohita against Aeromonas hydrophila

• Published in: Journal of immunology research Vol. 2015; no. 2015; pp. 1 - 10
• Main Authors: Sukumaran, V., Yun, Saekil, Kim, Hyoun Joong, Chi, Cheng, Sen, Shib Sankar, Giri, Sib Sankar, Park, Se Chang
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2015; Hindawi Limited
• Subjects: Aeromonas hydrophila; Aeromonas hydrophila - immunology; Aeromonas hydrophila - pathogenicity; Animal Feed; Animals; Carp; Complement C3 - genetics; Cyprinidae; Dietary Supplements; Disease Resistance - drug effects; Fish; Fish Diseases - diet therapy; Fish Diseases - immunology; Fish Diseases - microbiology; Fish Diseases - mortality; Gene Expression Regulation; Gram-Negative Bacterial Infections - diet therapy; Gram-Negative Bacterial Infections - immunology; Gram-Negative Bacterial Infections - mortality; Gram-Negative Bacterial Infections - veterinary; Hogs; Immunity, Innate - drug effects; Immunology; Interleukin-10 - genetics; Interleukin-10 - immunology; Interleukin-1beta - genetics; Interleukin-1beta - immunology; Interleukin-8 - genetics; Interleukin-8 - immunology; Labeo rohita; Liliaceae - chemistry; Muramidase - genetics; Muramidase - immunology; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - immunology; Phagocytosis - drug effects; Pneumoviridae; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Respiratory Burst - drug effects; Studies; Survival Analysis; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - immunology; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology
• ISSN: 2314-8861; 2314-7156
• DOI: 10.1155/2015/256510

The present study aimed to investigate the effects of Chlorophytum borivilianum polysaccharide (CBP), as a dietary supplement administered at varying concentrations with feed (basal diet), on various cytokine-related responses in Labeo rohita fingerlings. Immune parameters and immune-related gene expressions were measured at 3rd, 4th, and 5th week after feeding. The results revealed that dietary administration of CBP at 0.2% and 0.4% for 4 weeks significantly upregulated serum lysozyme and phagocytic activity. Complement C3 and respiratory burst activity (RBA) were significantly higher after 4 weeks of CBP feeding. The immune related genes IL-8, IL-1β, TNF-α, and iNOS were downregulated (P<0.05) in groups with 0.2% and 0.4% CBP supplemented diets at week 4. Expression of anti-inflammatory cytokines (IL-10 and TGF-β) was also downregulated (P<0.5) after 4 weeks of feeding with 0.2% to 0.8% CBP. However, five weeks of CBP administration had no significant effect on immune gene expression, except TNF-α and IL-8. Fish fed with 0.4% CBP for 4 weeks showed maximum resistance against Aeromonas hydrophila (73.3% survival) compared to control. From these results, we recommend that CBP administration at 0.4% for 4 weeks could effectively improve immune response and disease resistance in L. rohita.