Role of IQGAP3 in metastasis and epithelial-mesenchymal transition in human hepatocellular carcinoma

• Published in: Journal of translational medicine Vol. 15; no. 1; p. 176
• Main Authors: Shi, Yongjie, Qin, Nan, Zhou, Qiang, Chen, Yanqiu, Huang, Sicong, Chen, Bo, Shen, Gang, Jia, Hongyun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15-08-2017; BioMed Central; BMC
• Subjects: Animal tissues; Animals; Bioindicators; Bone marrow; Breast cancer; Cancer metastasis; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; Cell adhesion & migration; Cell Line, Tumor; Cell lines; Cell migration; Cell Movement - genetics; Classification; Cytoskeleton; Development and progression; Ectopic expression; Epithelial cells; Epithelial-Mesenchymal Transition - genetics; Epithelial–mesenchymal transition; Female; Gastroenterology; Gene amplification; Gene expression; Gene Expression Regulation, Neoplastic; Growth factors; GTPase-Activating Proteins - genetics; GTPase-Activating Proteins - metabolism; Guanosine triphosphatases; Health aspects; Hepatocellular carcinoma; Hepatocytes; Hepatology; Humans; Immunohistochemistry; Invasiveness; IQGAP3; Kinases; Large intestine; Liver; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - pathology; Lungs; Male; Medical prognosis; Mesenchyme; Metastases; Metastasis; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Migration; Mortality; mRNA; Multivariate Analysis; Neoplasm Invasiveness; Neoplasm Metastasis; Observations; Polymerase chain reaction; Prognosis; Proteins; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal transduction; Signal Transduction - genetics; Statistics, Nonparametric; TGF-β signaling; Tissues; Transcription factors; Transforming Growth Factor beta - metabolism; Tumors; Up-Regulation - genetics; Wound healing; China; Epithelial–mesenchymal transition; Hepatocellular carcinoma; Metastasis; IQGAP3; TGF-β signaling
• ISSN: 1479-5876; 1479-5876
• DOI: 10.1186/s12967-017-1275-8

Hepatocellular carcinoma (HCC) is one of the most lethal cancers worldwide owing to its high rates of metastasis and recurrence. The oncogene IQ motif-containing GTPase activating protein 3 (IQGAP3) is ubiquitously overexpressed in several human cancers, including liver, ovary, lung, large intestine, gastric, bone marrow, and breast malignancies and is involved in the invasion and metastasis of cancer cells. Therefore, we aimed to determine the biological role and molecular mechanism of IQGAP3 in HCC.
We used 120 archived clinical HCC samples, 9 snap-frozen HCC tumor tissues, and 4 normal liver tissues. Expression of IQGAP3 mRNA and protein in HCC cell lines (Hep3B, SMMC-7721, HCCC-9810, HepG2, BEL-7404, HCCLM3, QGY-7701, Huh7, and MHCC97H) and normal liver epithelial cells LO2 was examined by western blot, quantitative polymerase chain reaction, and immunohistochemistry. In addition, wound-healing and transwell matrix penetration assays were used to assess the migratory and invasive abilities of HCC cells, respectively.
Expression of the IQGAP3 was robustly upregulated in HCC cells and tissues. High expression of IQGAP3 in HCC correlated with aggressive clinicopathological features and was an independent poor prognostic factor for overall survival. Furthermore, ectopic expression of IQGAP3 markedly enhanced HCC cell migration, invasion, and epithelial-to-mesenchymal transition (EMT) in vitro and promoted metastasis of orthotopic hepatic tumors in nude mice. Conversely, silencing endogenous IQGAP3 showed an opposite effect. Mechanistically, IQGAP3 promoted EMT and metastasis by activating TGF-β signaling.
IQGAP3 functions as an important regulator of metastasis and EMT by constitutively activating the TGF-β signaling pathway in HCC. Our findings present new evidence of the role of IQGAP3 in EMT and metastasis, indicating its potential as a prognostic biomarker candidate and a therapeutic target against HCC.