Anti-inflammatory function of apolipoprotein B-depleted plasma is impaired in non-alcoholic fatty liver disease

• Published in: PloS one Vol. 17; no. 4; p. e0266227
• Main Authors: Sarmadi, Negar, Poustchi, Hossein, Ali Yari, Fatemeh, Radmard, Amir Reza, Karami, Sara, Pakdel, Abbas, Shabani, Parisa, Khaleghian, Ali
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12-04-2022; Public Library of Science (PLoS)
• Subjects: Adhesion; Anti-inflammatory agents; Anti-Inflammatory Agents - blood; Apolipoprotein B; Apolipoproteins; Apolipoproteins B - blood; Biochemistry; Biology and Life Sciences; Blood pressure; Cardiovascular diseases; Cardiovascular system; Care and treatment; Carotid arteries; Case-Control Studies; Cell adhesion molecules; Cholesterol; Complications and side effects; Coronary vessels; Cross-Sectional Studies; Depletion; Diabetes; Endothelial cells; Fasting; Fatty liver; Gene expression; Health aspects; Health risks; Hepatitis; High density lipoprotein; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Inflammation - blood; Liver; Liver diseases; Medicine; Medicine and Health Sciences; Metabolic syndrome; Non-alcoholic Fatty Liver Disease - blood; Obesity; Patient outcomes; Physiological aspects; Plasma; Risk factors; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Ultrasonic imaging; Umbilical vein; Iran; United States
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0266227

Non-alcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular events. HDL exerts various protective functions on the cardiovascular system including anti-inflammatory activity by suppressing adhesion molecules expression in inflammation-induced endothelial cells. This study was designed to search if the anti-inflammatory capacity of apolipoprotein B-depleted plasma (apoB-depleted plasma) is altered in NAFLD patients.
A total of 83 subjects including 42 NAFLD and 41 control subjects were included in this cross-sectional study. Anti-inflammatory function of HDL was determined as the ability of apoB-depleted plasma to inhibit tumor necrosis factor-α (TNF-α)-induced expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs).
Incubation of inflammation-stimulated HUVECs with the NAFLD patients' apo-B depleted plasma led to higher levels of expression of adhesion molecules compared to the control subjects' plasma samples, reflecting an impaired anti-inflammatory capacity of apoB-depleted plasma in the NAFLD patients. Impaired anti-inflammatory capacity of apoB-depleted plasma was correlated with fatty liver and obesity indices. After adjustment with obesity indices, the association of anti-inflammatory capacity of apoB-depleted plasma with NAFLD remained significant.
Impaired anti-inflammatory activity of apoB-depleted plasma was independently associated with NAFLD.