A genome-wide association study on fish consumption in a Japanese population—the Japan Multi-Institutional Collaborative Cohort study

• Published in: European journal of clinical nutrition Vol. 75; no. 3; pp. 480 - 488
• Main Authors: Suzuki, Taro, Nakamura, Yasuyuki, Matsuo, Keitaro, Oze, Isao, Doi, Yukio, Narita, Akira, Shimizu, Atsushi, Imaeda, Nahomi, Goto, Chiho, Matsui, Kenji, Nakatochi, Masahiro, Miura, Katsuyuki, Takashima, Naoyuki, Kuriki, Kiyonori, Shimanoe, Chisato, Tanaka, Keitaro, Ikezaki, Hiroaki, Murata, Masayuki, Ibusuki, Rie, Takezaki, Toshiro, Koyanagi, Yuriko, Ito, Hidemi, Matsui, Daisuke, Koyama, Teruhide, Mikami, Haruo, Nakamura, Yohko, Suzuki, Sadao, Nishiyama, Takeshi, Katsuura-Kamano, Sakurako, Arisawa, Kokichi, Takeuchi, Kenji, Tamura, Takashi, Okada, Rieko, Kubo, Yoko, Momozawa, Yukihide, Kubo, Michiaki, Kita, Yoshikuni, Wakai, Kenji
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01-03-2021; Nature Publishing Group
• Subjects: 45/43; 631/181/457/649; 631/208; Aldehyde dehydrogenase; Chromosome 12; Chromosome 14; Chromosomes; Clinical Nutrition; Cohort analysis; Collaboration; Consumption data; Drinking behavior; Energy intake; Environmental factors; Epidemiology; Fish; Fish as food; Food intake; Genetic aspects; Genetic diversity; Genetic factors; Genome-wide association studies; Genomes; Health aspects; Internal Medicine; Medicine; Medicine & Public Health; Metabolic Diseases; Nucleotides; Principal components analysis; Public Health; Regression analysis; Regression models; Replication; Single-nucleotide polymorphism; Japan
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/s41430-020-00702-7

Background/objective
Although benefits of fish consumption for health are well known, a significant percentage of individuals dislike eating fish. Fish consumption may be influenced by genetic factors in addition to environmental factors. We conducted a genome-wide association study (GWAS) to find genetic variations that affect fish consumption in a Japanese population.
Methods
We performed a two-stage GWAS on fish consumption using 13,739 discovery samples from the Japan Multi-Institutional Collaborative Cohort study, and 2845 replication samples from the other population. We used a semi-quantitative food frequency questionnaire to estimate food intake. Association of the imputed variants with fish consumption was analyzed by separate linear regression models per variant, with adjustments for age, sex, energy intake, principal component analysis components 1–10, and alcohol intake (g/day). We also performed conditional analysis.
Results
We found 27 single nucleotide polymorphisms (SNPs) located in 12q24 and 14q32.12 that were associated with fish consumption. The 19 SNPs were located at 11 genes including six lead SNPs at the
BRAP, ACAD10, ALDH2, NAA25, and HECTD4
regions on 12q24.12-13, and
CCDC197
region on 14q32.12. In replication samples, all five SNPs located on chromosome 12 were replicated successfully, but the one on chromosome 14 was not. Conditional analyses revealed that the five lead variants in chromosome 12 were in fact the same signal.
Conclusion
We found that new SNPs in the 12q24 locus were related to fish intake in two Japanese populations. The associations between SNPs on chromosome 12 and fish intake were strongly confounded by drinking status.