Whole-Genome Analysis of Bartonella ancashensis, a Novel Pathogen Causing Verruga Peruana, Rural Ancash Region, Peru

• Published in: Emerging infectious diseases Vol. 23; no. 3; pp. 430 - 438
• Main Authors: Mullins, Kristin E, Hang, Jun, Clifford, Robert J, Onmus-Leone, Fatma, Yang, Yu, Jiang, Ju, Leguia, Mariana, Kasper, Matthew R, Maguina, Ciro, Lesho, Emil P, Jarman, Richard G, Richards, Allen, Blazes, David
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01-03-2017; Centers for Disease Control and Prevention
• Subjects: a Novel Human Pathogen Causing Verruga Peruana, Rural Ancash Region, Peru; Adolescent; Adult; Analysis; bacteria; Bartonella; Bartonella - classification; Bartonella - genetics; Bartonella Infections - epidemiology; Bartonella Infections - microbiology; Bartonellosis; Child; Child, Preschool; Female; Genome, Bacterial; Genomes; Genomics; Humans; Infant; Male; Middle Aged; novel phylogenetic lineage; Peru - epidemiology; Phylogeny; Proteins; rural Ancash region; verruga peruana; whole-genome analysis; Whole-Genome Analysis of; Young Adult; Maryland; Bartonella; Bartonella ancashensis; verruga peruana; bacteria; bartonellosis; rural Ancash region; Bartonella bacilliformis; Peru; whole-genome analysis; novel phylogenetic lineage
• ISSN: 1080-6040; 1080-6059
• DOI: 10.3201/eid2303.161476

The genus Bartonella contains >40 species, and an increasing number of these Bartonella species are being implicated in human disease. One such pathogen is Bartonella ancashensis, which was isolated in blood samples from 2 patients living in Caraz, Peru, during a clinical trial of treatment for bartonellosis. Three B. ancashensis strains were analyzed by using whole-genome restriction mapping and high-throughput pyrosequencing. Genome-wide comparative analysis of Bartonella species showed that B. ancashensis has features seen in modern and ancient lineages of Bartonella species and is more related to B. bacilliformis. The divergence between B. ancashensis and B. bacilliformis is much greater than what is seen between known Bartonella genetic lineages. In addition, B. ancashensis contains type IV secretion system proteins, which are not present in B. bacilliformis. Whole-genome analysis indicates that B. ancashensis might represent a distinct Bartonella lineage phylogenetically related to B. bacilliformis.