Insights into anaphase promoting complex TPR subdomain assembly from a CDC26-APC6 structure

• Published in: Nature structural & molecular biology Vol. 16; no. 9; pp. 987 - 989
• Main Authors: Schulman, Brenda A, Rajashankar, Kanagalaghatta R, Wang, Jing, Dye, Billy T, Kurinov, Igor
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01-09-2009; Nature Publishing Group
• Subjects: Amino Acid Motifs; Amino Acid Sequence; Anaphase; Anaphase-Promoting Complex-Cyclosome; Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; BASIC BIOLOGICAL SCIENCES; Biochemistry; Biological Microscopy; Biomedical and Life Sciences; Biophysics; brief-communication; CELL CYCLE; Cell Cycle Proteins - chemistry; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cellular proteins; Circular Dichroism; GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; Genetic aspects; GENETICS; Humans; Life Sciences; Membrane Biology; MITOSIS; Models, Molecular; Molecular biology; Molecular Sequence Data; Physiological aspects; Protein Binding; Protein Structure; Protein Structure, Quaternary; Protein Structure, Tertiary; PROTEINS; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sequence Alignment; Structure; Ubiquitin-Protein Ligase Complexes - chemistry; Ubiquitin-Protein Ligase Complexes - genetics; Ubiquitin-Protein Ligase Complexes - metabolism; Ubiquitin-Protein Ligases - chemistry; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Yeast fungi; United States
• ISSN: 1545-9993; 1545-9985
• DOI: 10.1038/nsmb.1645

The multisubunit anaphase promoting complex (APC) is an essential cell-cycle regulator. Although CDC26 is known to have a role in APC assembly, its molecular function has remained unclear. Biophysical, structural and genetic studies presented here reveal that CDC26 stabilizes the structure of APC6, a core TPR protein required for APC integrity. Notably, CDC26-APC6 association involves an intermolecular TPR mimic composed of one helix from each protein.