Egg-adaptive mutations in H3N2v vaccine virus enhance egg-based production without loss of antigenicity or immunogenicity

Highlights • We passaged variant influenza A H3N2v virus in eggs to induce egg-adaptive HA mutations. • PR8-based 6 + 2 candidate vaccine viruses (CVVs) with the HA mutations were characterized. • Four sets of HA substitutions retained their immunogenicity and produced high-yield HA. • The HA substi...

Full description

Saved in:

Bibliographic Details
Published in:	Vaccine Vol. 33; no. 28; pp. 3186 - 3192
Main Authors:	Barman, Subrata, Franks, John, Turner, Jasmine C, Yoon, Sun-Woo, Webster, Robert G, Webby, Richard J
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 22-06-2015 Elsevier Limited
Subjects:	Adaptation, Physiological Allergy and Immunology Amino Acid Substitution Animals Antibodies, Viral - immunology Antigenicity Binding sites Blood Chick Embryo Cloning Egg adaptation Eggs Ferrets Genetics Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin H3 mutations Humans Immunogenicity Influenza Influenza A virus Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - growth & development Influenza A Virus, H1N1 Subtype - immunology Influenza A Virus, H1N1 Subtype - metabolism Influenza A Virus, H3N2 Subtype - genetics Influenza A Virus, H3N2 Subtype - growth & development Influenza A Virus, H3N2 Subtype - immunology Influenza A Virus, H3N2 Subtype - metabolism Laboratories Male Mutation Proteins Serial Passage Sucrose Swine Vaccine virus Vaccines Variant virus Virus Cultivation Virus Replication Viruses Zoonosis CVV Egg adaptation Madin-Darby canine kidney cells plaque forming unit H3N2v Zoonosis Vaccine virus CRBC H3N2 variant Influenza A virus Antigenicity Immunogenicity TRBC Variant virus MDCK candidate vaccine virus chicken red blood cells PFU Hemagglutinin H3 mutations turkey red bold cells
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Highlights • We passaged variant influenza A H3N2v virus in eggs to induce egg-adaptive HA mutations. • PR8-based 6 + 2 candidate vaccine viruses (CVVs) with the HA mutations were characterized. • Four sets of HA substitutions retained their immunogenicity and produced high-yield HA. • The HA substitutions G186V + N246 K appear optimal for egg-based H3N2v vaccine production.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2015.05.011