Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics

• Published in: FEBS letters Vol. 547; no. 1; pp. 151 - 156
• Main Authors: Wong, Joshua H, Balmer, Yves, Cai, Nick, Tanaka, Charlene K, Vensel, William H, Hurkman, William J, Buchanan, Bob B
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 17-07-2003
• Subjects: Conserved Sequence; Cysteine; DTT, dithiothreitol; Endosperm; GSH, reduced glutathione; mBBr, monobromobimane; Oxidative Stress; PDI, protein disulfide isomerase; PFP, fructose 6-phosphate 1-phosphotransferase; plant proteins; Plant Proteins - chemistry; Plant Proteins - isolation & purification; Plant Proteins - metabolism; Plant Structures - metabolism; PPDK, pyruvate,Pi dikinase; PPi, inorganic pyrophosphate; protein metabolism; Proteomics; Redox regulation; seeds; Seeds - metabolism; Thioredoxin; Thioredoxins - chemistry; Thioredoxins - isolation & purification; Thioredoxins - metabolism; Triticum - metabolism; Triticum aestivum; Wheat; GSH, reduced glutathione; PPi, inorganic pyrophosphate; Redox regulation; Thioredoxin; PFP, fructose 6-phosphate 1-phosphotransferase; DTT, dithiothreitol; PDI, protein disulfide isomerase; Proteomics; PPDK, pyruvate,Pi dikinase; Endosperm; mBBr, monobromobimane; Wheat; Triticum aestivum
• ISSN: 0014-5793; 1873-3468; 1873-3468
• DOI: 10.1016/S0014-5793(03)00696-3

Application of a thiol-specific probe, monobromobimane, with proteomics and enzyme assays led to the identification of 23 thioredoxin targets in the starchy endosperm of mature wheat seeds (
Triticum aestivum cv. Butte), almost all containing at least two conserved cysteines. The identified targets, 12 not known to be thioredoxin-linked, function in a spectrum of processes: metabolism (12 targets), protein storage (three), oxidative stress (three), protein degradation (two), protein assembly/folding (one) and unknown reactions (two). In addition to formulating metabolic pathways functional in the endosperm, the results suggest that thioredoxin acts in redox regulation throughout the life cycle of the seed.