Cloning and characterization of a 23-kDa stress-induced mouse peritoneal macrophage protein

• Published in: The Journal of biological chemistry Vol. 268; no. 25; pp. 18633 - 18636
• Main Authors: Ishii, T., Yamada, M., Sato, H., Matsue, M., Taketani, S., Nakayama, K., Sugita, Y., Bannai, S.
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05-09-1993; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Animals; Arsenic - pharmacology; Arsenites; Base Sequence; Biological and medical sciences; Blotting, Northern; Cell physiology; Cells, Cultured; Cloning, Molecular; DNA - chemistry; DNA - isolation & purification; Effects of physical and chemical agents; Fundamental and applied biological sciences. Psychology; Heat-Shock Proteins - biosynthesis; Heat-Shock Proteins - chemistry; Heat-Shock Proteins - genetics; Hydrogen Peroxide - pharmacology; Immunoblotting; Immunosorbent Techniques; Macrophages - drug effects; Macrophages - metabolism; Maleates - pharmacology; Mice; Molecular and cellular biology; Molecular Sequence Data; Molecular Weight; Nucleic Acid Hybridization; Peritoneal Cavity - cytology; Peroxidases; Peroxiredoxins; Protein Biosynthesis; RNA, Messenger - analysis; RNA, Messenger - metabolism; Sodium Compounds; Tissue Distribution; Proteins; Vertebrata; Mammalia; Mouse; Transcription; Induction; Rodentia; Primary structure; Oxidation; Stress; Macrophage
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)46676-2

Exposure of mouse peritoneal macrophages to oxidative and sulfhydryl-reactive agents in vitro enhances synthesis of a few cellular proteins that may be important in a self-defense system. A cDNA encoding a novel stress-inducible protein, designated MSP23 (macrophage 23-kDa stress protein), was cloned from a cDNA library of the macrophages by differential screening. A 1.0-kilobase mRNA transcript hybridized with the MSP23 cDNA gradually increased in macrophages upon culture in vitro. Treatment with diethylmaleate or glucose/glucose oxidase, which generates H2O2, markedly enhanced the induction of the transcript after several hours. Cadmium chloride and sodium arsenite also induced the transcript. An antiserum raised against recombinant MSP23 reacted with the 23-kDa stress-inducible protein of the macrophages. The amounts of 23-kDa protein in the cells rapidly increased during culture with diethylmaleate. The mRNA was detected in various tissues, and it was especially high in content in the liver. A search of databases revealed that six proteins of various species from bacteria to the mouse have a sequence homology to MSP23. One of the proteins is the C22 component of alkyl hydroperoxide reductase, which is induced by hydrogen peroxide in Salmonella typhimurium.