Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats

• Published in: Brazilian journal of medical and biological research Vol. 54; no. 11; pp. 1 - e11352
• Main Authors: Luna, G.L. Flores, Oehlmeyer, T.L, Brandao, G, Brassolatti, P, Tosta, J, Goto, L.S, de Avo, L, de Oliveira Leal, A.M
• Format: Journal Article
• Language: English
• Published: Ribeirao Preto Associacao Brasileira de Divulgacao Cientifica (ABDC) 01-01-2021; Revista Brasileira de Pesquisas Medicas; Associação Brasileira de Divulgação Científica
• Subjects: Animal model; BIOLOGY; Birefringence; Bone marrow; Care and treatment; Cell therapy; Collagen; Diabetes; Diabetes mellitus; Health aspects; Lesions; MEDICINE, RESEARCH & EXPERIMENTAL; Mesenchymal stem cells; Rodents; Stem cell research; Stem cell transplantation; Stem cells; Streptozocin; Telomerase; Transplantation; Wound; Wound healing; Wounds and injuries; Animal model; Cell therapy; Wound; Diabetes mellitus; Mesenchymal stem cells
• ISSN: 0100-879X; 1414-431X; 1414-431X; 1678-4510
• DOI: 10.1590/1414-431X2021e11352

Diabetes mellitus is associated with neural and micro- and macrovascular complications. Therapeutic options for these complications are limited and the delivery of mesenchymal stem cells into lesions have been reported to improve the healing process. In this work, the effects of the administration of a lineage of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase (hBMSC-TERT) as a potential therapeutic tool for wound healing in diabetic rats were investigated. This is the first description of the use of these cells in diabetic wounds. Dorsal cutaneous lesions were made in streptozotocin-induced diabetic rats and hBMSC-TERT were subcutaneously administered around the lesions. The healing process was evaluated macroscopically, histologically, and by birefringence analysis. Diabetic wounded rats infused with hBMSC-TERT (DM-TERT group) and the non-diabetic wounded rats not infused with hBMSC-TERT (CW group) had very similar patterns of fibroblastic response and collagen proliferation indicating improvement of wound healing. The result obtained by birefringence analysis was in accordance with that obtained by the histological analysis. The results indicated that local administration of hBMSC-TERT in diabetic wounds improved the wound healing process and may become a therapeutic option for wounds in individuals with diabetes. Key words: Cell therapy; Diabetes mellitus; Mesenchymal stem cells; Wound; Animal model