Loss of HLA-DR Expression by Human Epidermal Cells After Growth in Culture

• Published in: Journal of investigative dermatology Vol. 83; no. 1; pp. 48 - 50
• Main Authors: Hefton, John M., Amberson, James B., Biozes, David G., Weksler, Marc E.
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01-07-1984; Nature Publishing
• Subjects: Animal cells; Antigens, Differentiation, Myelomonocytic; Antigens, Surface - analysis; Biological and medical sciences; Cell culture; Cell cultures. Hybridization. Fusion; Cell proliferation; Cells, Cultured; Dermatology; Epidermis - ultrastructure; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Histocompatibility antigen HLA; Histocompatibility Antigens Class II - analysis; HLA-DR Antigens; Humans; Kinetics; Lymphocytes T; Microscopy, Electron; Molecular and cellular biology; Skin; Skin - immunology; T-Lymphocytes - immunology; Transplantation Immunology; Transplantation, Homologous; Human; Cell culture; Skin; Immunocytochemistry
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1111/1523-1747.ep12261671

These studies present evidence that when human epidermal cells are grown in culture they lose both the ability to stimulate the proliferation of allogeneic T lymphocytes in vitro and their expression of HLA-DR antigens. Our results also show that epidermal cells incubated with anti-HLA-DR serum lose their ability to stimulate the proliferation to allogeneic T lymphocytes in a mixed skin cell-lymphocyte reaction.