Protective Effect of Detoxified Rhus verniciflua Stokes on Human Keratinocytes and Dermal Fibroblasts against Oxidative Stress and Identification of the Bioactive Phenolics

• Published in: Bioscience, biotechnology, and biochemistry Vol. 77; no. 8; pp. 1682 - 1688
• Main Authors: LIU, Chun-Shan, NAM, Tae-Gyu, HAN, Min-Woo, AHN, Soo-mi, CHOI, Han Seok, KIM, Tae Young, CHUN, Ock K., KOO, Sung I., KIM, Dae-Ok
• Format: Journal Article
• Language: English
• Published: England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013; Oxford University Press
• Subjects: Cell Line; Fibroblasts - drug effects; Fibroblasts - radiation effects; Gene Expression Regulation - drug effects; Humans; Hydrogen Peroxide - toxicity; Keratinocytes - drug effects; Keratinocytes - radiation effects; Matrix Metalloproteinase 1 - biosynthesis; matrix metalloproteinase-1; Oxidative Stress - drug effects; Oxidative Stress - radiation effects; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Reactive Oxygen Species; Rhus; skin aging; Tandem Mass Spectrometry; Toxicodendron - chemistry; ultraviolet A; Ultraviolet Rays; vitamin C equivalent antioxidant capacity
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.130236

Oxidative stress due to the over-production of reactive oxygen species (ROS) is associated with human skin aging. This study was designed to identify the bioactive phenolics in detoxified Rhus verniciflua Stokes (DRVS) that may protect human skin against oxidative stress. Under oxidative stress caused by H
2
O
2
, the 40% (v/v) aqueous methanol extract of DRVS protected human keratinocytes in a dose-dependent manner. The expression of matrix metalloproteinase-1 (MMP-1) was also inhibited by the DRVS extract in human dermal fibroblasts-neonatal cells exposed to ultraviolet A. The major bioactive phenolics of DRVS were tentatively identified by LC/Q-TOF-ESI-MS/MS, and included gallic acid, 2-(ethoxymethoxy)-3-hydroxyphenol, fustin, a fustin isomer, tetragalloyl glucose, pentagalloyl glucose, fisetin, sulfuretin, a sulfuretin isomer, and butein. The results suggest that a DRVS extract may be effective in slowing skin aging through its antioxidative properties and by down-regulating MMP-1 expression. Further studies are needed to examine whether this effect would be mediated by the phenolics identified in this study.