Molecular characterization of Helicobacter pylori strains isolated from cynomolgus monkeys ( M. fascicularis)

• Published in: Veterinary microbiology Vol. 108; no. 1; pp. 133 - 139
• Main Authors: Doi, Sonia Q., Kimbason, Tara, Reindel, James, Dubois, Andre
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-06-2005; Elsevier Science
• Subjects: 16S ribosomal RNA; animal diseases; Animal model; animal pathogenic bacteria; Animals; bacterial infections; Bacteriology; Biological and medical sciences; Cynomolgus monkey; DNA Fingerprinting; DNA sequencing; Fundamental and applied biological sciences. Psychology; Gastric erosions; Gastritis; Genotype; Genotyping; geographical variation; Helicobacter Infections - microbiology; Helicobacter Infections - veterinary; Helicobacter pylori; Helicobacter pylori - genetics; Helicobacter pylori - isolation & purification; Macaca fascicularis; Macaca fascicularis - microbiology; Microbiology; Miscellaneous; molecular sequence data; Monkey Diseases - microbiology; Nonhuman primate; nucleotide sequences; ribosomal RNA; RNA, Bacterial - chemistry; RNA, Ribosomal, 16S - chemistry; Sequence Alignment; Sequence Homology, Nucleic Acid; Stomach; strain differences; strains; Vietnam; Philippines; Stomach; Animal model; Helicobacter pylori; Genotyping; 16S ribosomal RNA; Nonhuman primate; Cynomolgus monkey; Gastric erosions; DNA fingerprinting; Gastritis; DNA sequencing; Microbiology; Spirillaceae; Macaca fascicularis; Ribosomal RNA; Primates; Simioidea; 16S-RNA; Bacteria; Sequencing; Gastric disease; Typing; Spirillales; Genotype; Strain; Vertebrata; Mammalia; Fingerprint method; Digestive diseases; Veterinary
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2005.04.003

We recently reported the occurrence of natural infection with
H. pylori in a group of cynomolgus monkeys with chronic active gastritis and gastric erosions. The goal of the present study was to characterize and to compare strains isolated from animals originating from two different geographical areas. Gross and microscopic pathology determined at the time of necropsy was similar in all animals.
H. pylori were isolated from specimens harvested in five monkeys (four from Vietnam and one from the Philippines) with gastritis. Isolates from monkeys bred in Vietnam had a similar DNA fingerprint pattern, which was distinct from that of isolates from a monkey bred in the Philippines. All strains were of the
s1a vacA subtype, but all the ‘Vietnamese’ strains were
cagA
+ and all but one were
iceA1 whereas the ‘Philippino’ strains were
cagA
− and
iceA2. The sequences of the 16S rRNA of the Vietnamese and Philippino strains shared 98% homology and both clustered with
H. pylori sequences present in the NCBI database. In conclusion, cynomolgus monkeys can be naturally colonized by
H. pylori, and the strains isolated from these animals appear to vary according to the geographical origin, thus indicating probable infection prior to importation. Since some of the cynomolgus monkeys developed antral erosions during natural infection, we propose that this animal model may be used to investigate the role of
H. pylori in ulcerogenesis.