Effects of X-radiation on lung cancer cells: the interplay between oxidative stress and P53 levels

• Published in: Medical oncology (Northwood, London, England) Vol. 32; no. 12; p. 266
• Main Authors: Mendes, Fernando, Sales, Tiago, Domingues, Cátia, Schugk, Susann, Abrantes, Ana Margarida, Gonçalves, Ana Cristina, Teixo, Ricardo, Silva, Rita, Casalta-Lopes, João, Rocha, Clara, Laranjo, Mafalda, Simões, Paulo César, Sarmento Ribeiro, Ana Bela, Botelho, Maria Filomena, Rosa, Manuel Santos
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2015; Springer Nature B.V
• Subjects: Apoptosis - radiation effects; Cell Line, Tumor; Hematology; Humans; Internal Medicine; Lung cancer; Lung Neoplasms - radiotherapy; Medicine; Medicine & Public Health; Models, Biological; Oncology; Original Paper; Oxidative Stress - radiation effects; Pathology; Tumor Suppressor Protein p53 - analysis; Tumor Suppressor Protein p53 - metabolism; X-Rays; Oxidative stress; Radiotherapy; Lung cancer; P53
• ISSN: 1357-0560; 1559-131X
• DOI: 10.1007/s12032-015-0712-x

Lung cancer (LC) ranks as the most prevalent and deadliest cause of cancer death worldwide. Treatment options include surgery, chemotherapy and/or radiotherapy, depending on LC staging, without specific highlight. The aim was to evaluate the effects of X-radiation in three LC cell lines. H69, A549 and H1299 cell lines were cultured and irradiated with 0.5–60 Gy of X-radiation. Cell survival was evaluated by clonogenic assay. Cell death and the role of reactive oxygen species, mitochondrial membrane potential, BAX, BCL-2 and cell cycle were analyzed by flow cytometry. Total and phosphorylated P53 were assessed by western blotting. Ionizing radiation decreases cell proliferation and viability in a dose-, time- and cell line-dependent manner, inducing cell death preferentially by apoptosis with cell cycle arrest. These results may be related to differences in P53 expression and oxidative stress response. The results obtained indicate that sensibility and/or resistance to radiation may be dependent on molecular LC characteristics which could influence response to radiotherapy and treatment success.