Degradation of chlorophenols by Phanerochaete chrysosporium: effect of 3,4-dichlorophenol on extracellular peroxidase activities

• Published in: Applied microbiology and biotechnology Vol. 59; no. 2/3; pp. 284 - 288
• Main Authors: Duran, R, Deschler, C, Precigou, S, Goulas, P
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-07-2002; Springer Nature B.V; Springer Verlag
• Subjects: Activation; Agitated; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Blotting, Northern; Chemical Sciences; Chlorophenols; Chlorophenols - metabolism; Chlorophenols - pharmacology; Culture; Degradation; Fundamental and applied biological sciences. Psychology; Fungi; Inoculation; Lignin; lignin peroxidase; Manganese; manganese peroxidase; messenger RNA; metabolism; microbial growth; Mission oriented research; Northern blotting; Peroxidase; Peroxidases - drug effects; Peroxidases - genetics; Peroxidases - metabolism; Phanerochaete - metabolism; Phanerochaete chrysosporium; Physiology and metabolism; proteinases; transcription (genetics); Fungi; Biodegradation; Pollutant; Peroxidases; Enzyme; Chlorine Organic compounds; Phanerochaete chrysosporium; Phenols; Basidiomycetes; Oxidoreductases; Extracellular; Thallophyta
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-002-0988-6

Extracellular peroxidases play an important role in the degradation of chlorophenols by Phanerochaete chrysosporium. Depending on the moment of 3,4-dichlorophenol addition, the production of lignin peroxidase and manganese peroxidase in C-limited agitated cultures was affected in opposite ways. In cultures that received 3,4-dichlorophenol at the time of inoculation, fungal growth was reduced and peroxidases were not produced, whereas peroxidase activities were stabilized after 3,4-dichlorophenol addition to pregrown cultures. Further investigation revealed that mRNA encoding lignin peroxidase was not produced in cultures started with 3,4-dichlorophenol, suggesting that the onset of secondary metabolism was affected. In addition, the stabilization of lignin peroxidase activity was not the result of an activation of lignin peroxidase gene transcription, as shown by Northern blot experiments, but likely due to the inhibition of peroxidase degradation by extracellular proteases.