Large-scale identification of genes involved in septal pore plugging in multicellular fungi

• Published in: Nature communications Vol. 14; no. 1; p. 1418
• Main Authors: Mamun, Md. Abdulla Al, Cao, Wei, Nakamura, Shugo, Maruyama, Jun-ichi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17-03-2023; Nature Publishing Group; Nature Portfolio
• Subjects: 119/118; 14/19; 14/35; 631/326/193/2484; 631/326/193/2543; 631/326/88; 631/80; Ascomycota; Ascomycota - metabolism; Cytoplasm - metabolism; Divergence; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Genes; Genomes; Green Fluorescent Proteins - metabolism; Humanities and Social Sciences; Hyphae - metabolism; Localization; multidisciplinary; Plugging; Pores; Proteins; Science; Science (multidisciplinary); Septum; Wounding; Yeasts
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-36925-y

Multicellular filamentous fungi have septal pores that allow cytoplasmic exchange, and thus connectivity, between neighboring cells in the filament. Hyphal wounding and other stress conditions induce septal pore closure to minimize cytoplasmic loss. However, the composition of the septal pore and the mechanisms underlying its function are not well understood. Here, we set out to identify new septal components by determining the subcellular localization of 776 uncharacterized proteins in a multicellular ascomycete,
Aspergillus oryzae
. The set of 776 uncharacterized proteins was selected on the basis that their genes were present in the genomes of multicellular, septal pore-bearing ascomycetes (three
Aspergillus
species, in subdivision Pezizomycotina) and absent/divergent in the genomes of septal pore-lacking ascomycetes (yeasts). Upon determining their subcellular localization, 62 proteins were found to localize to the septum or septal pore. Deletion of the encoding genes revealed that 23 proteins are involved in regulating septal pore plugging upon hyphal wounding. Thus, this study determines the subcellular localization of many uncharacterized proteins in
A. oryzae
and, in particular, identifies a set of proteins involved in septal pore function.
Multicellular fungi have septal pores that allow cytoplasmic exchange between adjacent cells; cell wounding and other stress conditions induce septal pore closure. Here, Mamun et al. determine the subcellular localization of hundreds of uncharacterized proteins in a multicellular fungus, identifying 62 proteins associated with the septum. Of these, 23 proteins are involved in septal pore plugging upon hyphal wounding.