Y27632, a Rho-activated kinase inhibitor, normalizes dysregulation in alpha1-adrenergic receptor-induced contraction of Lyon hypertensive rat artery smooth muscle

• Published in: Fundamental & clinical pharmacology Vol. 23; no. 2; pp. 169 - 178
• Main Authors: Freitas, Maria Regina, Eto, Masumi, Kirkbride, Jason A., Schott, Christa, Sassard, Jean, Stoclet, Jean-Claude
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-04-2009
• Subjects: Amides - pharmacology; Animals; Ca2+ influx; Ca2+ sensitivity; Ca2+ signaling; Calcium - metabolism; Calcium Chloride - pharmacology; CPI-17; Disease Models, Animal; Hypertension - drug therapy; Hypertension - physiopathology; Lyon hypertensive rats; Male; Mesenteric Arteries - drug effects; Mesenteric Arteries - metabolism; Muscle Contraction - drug effects; Muscle Proteins - metabolism; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Myosin-Light-Chain Phosphatase - metabolism; MYPT1; Phosphoproteins - metabolism; Phosphorylation; Protein Kinase Inhibitors - pharmacology; Protein Kinase Inhibitors - therapeutic use; protein phosphatase; Protein Phosphatase 1 - metabolism; Pyridines - pharmacology; Rats; Receptors, Adrenergic, alpha-1 - metabolism; resistance mesentery artery; rho-Associated Kinases - antagonists & inhibitors; rho-Associated Kinases - metabolism; RhoA; ROK; Signal Transduction - drug effects; α1 adrenergic contraction
• ISSN: 0767-3981; 1472-8206
• DOI: 10.1111/j.1472-8206.2008.00658.x

RhoA‐activated kinase (ROK) is involved in the disorders of smooth muscle contraction found in hypertension model animals and patients. We examined whether the α1‐adrenergic receptor agonist‐induced ROK signal is perturbed in resistance small mesentery artery (SMA) of Lyon genetically hypertensive (LH) rats, using a ROK antagonist, Y27632. Smooth muscle strips of SMA and aorta were isolated from LH and Lyon normotensive (LN) rats. After Ca2+‐depletion and pre‐treatment with phenylephrine (PE), smooth muscle contraction was induced by serial additions of CaCl2. In LH SMA Ca2+ permeated cells to a lesser extent as compared with LN SMA, while CaCl2‐induced contraction of LH SMA was greater than that of LN SMA, indicating a higher ratio of force to Ca2+ in LH SMA contraction (Ca2+ sensitization). No hyper‐contraction was observed in LH aorta tissues. Treatment of LH SMA with Y27632 restored both Ca2+ permeability and Ca2+‐force relationship to levels seen for LN SMA. In response to PE stimulation, phosphorylation of CPI‐17, a phosphorylation‐dependent myosin phosphatase inhibitor protein, and MYPT1 at Thr853, the inhibitory phosphorylation site of the myosin phosphatase regulatory subunit, was increased in LN SMA, but remained unchanged in LH SMA. These results suggest that the disorder in ROK‐dependent Ca2+ permeability and Ca2+‐force relationship is responsible for LH SMA hyper‐contraction. Unlike other hypertensive models, the ROK‐induced hyper‐contractility of LH SMA is independent of MYPT1 and CPI‐17 phosphorylation, which suggests that ROK‐mediated inhibition of myosin phosphatase does not affect SMA hyper‐contractility in LH SMA cells.