Resveratrol possesses protective effects in a pristane-induced lupus mouse model

• Published in: PloS one Vol. 9; no. 12; p. e114792
• Main Authors: Wang, Zhuo-Long, Luo, Xiao-Fang, Li, Meng-Tao, Xu, Dong, Zhou, Shuang, Chen, Hou-Zao, Gao, Na, Chen, Zhen, Zhang, Ling-Ling, Zeng, Xiao-Feng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11-12-2014; Public Library of Science (PLoS)
• Subjects: Analysis; Animals; Antigens, CD - biosynthesis; Antigens, CD19 - metabolism; Antigens, Differentiation, T-Lymphocyte - biosynthesis; Apoptosis; Apoptosis - drug effects; Autoantibodies; Autoimmune diseases; Autoimmunity; B cells; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; B-Lymphocytes - pathology; Biology and Life Sciences; CD19 antigen; CD4 antigen; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; CD69 antigen; Cell proliferation; Chronic conditions; Cytometry; Flow cytometry; Gene Expression Regulation - drug effects; Glomerulonephritis; Helper cells; Humans; Immunoglobulin G; Immunomodulation; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - immunology; Kidneys; Lectins, C-Type - biosynthesis; Lupus; Lupus Erythematosus, Systemic - chemically induced; Lupus Erythematosus, Systemic - drug therapy; Lupus Erythematosus, Systemic - immunology; Lymphocytes; Lymphocytes B; Lymphocytes T; Medicine and Health Sciences; Mice; Pristane; Proteinuria; Receptors, Transferrin - biosynthesis; Research and Analysis Methods; Resveratrol; SIRT1 protein; Sirtuin 1 - deficiency; Sirtuin 1 - immunology; Spleen; Stilbenes - administration & dosage; Systemic lupus erythematosus; T cells; Terpenes - toxicity; γ-Interferon; Beijing China; United States > US; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0114792

Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by the production of autoantibodies. To date, no therapy has been found to satisfactorily treat SLE. SIRT1 deficiency results in the development of an autoimmune syndrome in mice, including a high titer of anti-nuclear antibody in serum, immunoglobulin deposition in the kidney, and immune complex glomerulonephritis. Resveratrol is an activator of SIRT1 and possesses anti-inflammation and immune-regulatory properties.
To evaluate the preventative effects of resveratrol on a pristane-induced lupus animal model and assess its putative immune modulation effects.
BALB/c mice received a single intraperitoneal injection of 0.5 ml of pristane on day 1 and then various doses of resveratrol were given to the mice daily starting on day 2 and continuing for seven months. The autoantibodies in serum and supernatants were measured. Single cells isolated from spleen, isolated CD4+ T cells, and CD19+ B cells were cultured with or without resveratrol in vitro and assessed by flow cytometry.
Resveratrol attenuated proteinuria, immunoglobuin depositon in kidney, and glomerulonephritis as well as IgG1 and IgG2a in serum in pristane-induced lupus mice. Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro. In vitro antibody production and proliferation of B cells were also inhibited.
Resveratrol possesses protective effects in pristane-induced lupus mice and may represent a novel approach for the management of SLE.