Analysis of Arabidopsis thaliana Redox Gene Network Indicates Evolutionary Expansion of Class III Peroxidase in Plants

• Published in: Scientific reports Vol. 9; no. 1; pp. 15741 - 9
• Main Authors: Oliveira, Raffael Azevedo de Carvalho, de Andrade, Abraão Silveira, Imparato, Danilo Oliveira, de Lima, Juliana Gabriela Silva, de Almeida, Ricardo Victor Machado, Lima, João Paulo Matos Santos, Pasquali, Matheus Augusto de Bittencourt, Dalmolin, Rodrigo Juliani Siqueira
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 31-10-2019; Nature Publishing Group; Nature Portfolio
• Subjects: 45; 631/181; 631/449/2669; 631/553/2716; Antioxidants; Antioxidants - chemistry; Antioxidants - metabolism; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Evolution; Evolution, Molecular; Gene Regulatory Networks - genetics; Humanities and Social Sciences; Metabolism; multidisciplinary; Orthology; Oxidation-Reduction; Oxidative metabolism; Peroxidase; Peroxidases - genetics; Peroxidases - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Reductase; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-52299-y

Reactive oxygen species (ROS) are byproducts of aerobic metabolism and may cause oxidative damage to biomolecules. Plants have a complex redox system, involving enzymatic and non-enzymatic compounds. The evolutionary origin of enzymatic antioxidant defense in plants is yet unclear. Here, we describe the redox gene network for
A. thaliana
and investigate the evolutionary origin of this network. We gathered from public repositories 246
A. thaliana
genes directly involved with ROS metabolism and proposed an
A. thaliana
redox gene network. Using orthology information of 238 Eukaryotes from STRINGdb, we inferred the evolutionary root of each gene to reconstruct the evolutionary history of
A. thaliana
antioxidant gene network. We found two interconnected clusters: one formed by SOD-related, Thiol-redox, peroxidases, and other oxido-reductase; and the other formed entirely by class III peroxidases. Each cluster emerged in different periods of evolution: the cluster formed by SOD-related, Thiol-redox, peroxidases, and other oxido-reductase emerged before opisthokonta-plant divergence; the cluster composed by class III peroxidases emerged after opisthokonta-plant divergence and therefore contained the most recent network components. According to our results, class III peroxidases are in expansion throughout plant evolution, with new orthologs emerging in each evaluated plant clade divergence.