Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores

• Published in: Nature communications Vol. 9; no. 1; pp. 4085 - 8
• Main Authors: Galenkamp, Nicole Stéphanie, Soskine, Misha, Hermans, Jos, Wloka, Carsten, Maglia, Giovanni
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05-10-2018; Nature Publishing Group; Nature Portfolio
• Subjects: 631/57/2265; 631/61/350/1058; 631/61/350/59; 9/97; Asparagine; Asparagine - analysis; Biosensing Techniques; Biosensors; Body fluids; Body Fluids - chemistry; Electronic devices; Electronic equipment; Escherichia coli; Glucose - analysis; Humanities and Social Sciences; Metabolites; Miniaturization; multidisciplinary; Nanopores; Porosity; Portable equipment; Proteins; Science; Science (multidisciplinary); Sensors; Signal Transduction; Sweat - chemistry
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-06534-1

Crucial steps in the miniaturisation of biosensors are the conversion of a biological signal into an electrical current as well as the direct sampling of bodily fluids. Here we show that protein sensors in combination with a nanopore, acting as an electrical transducer, can accurately quantify metabolites in real time directly from nanoliter amounts of blood and other bodily fluids. Incorporation of the nanopore into portable electronic devices will allow developing sensitive, continuous, and non-invasive sensors for metabolites for point-of-care and home diagnostics.
Protein nanopores are emerging as sensors for a variety of biomolecules. Here the authors develop a nanopore based on the bacterial toxin ClyA, in conjunction with binding proteins for glucose and asparagine, to detect these biomolecules simultaneously from a variety of unprocessed, diluted body fluids.