Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants

• Published in: Plant, cell and environment Vol. 31; no. 9; pp. 1237 - 1249
• Main Authors: OVERMYER, KIRK, KOLLIST, HANNES, TUOMINEN, HANNELE, BETZ, CHRISTIAN, LANGEBARTELS, CHRISTIAN, WINGSLE, GUNNAR, KANGASJÄRVI, SAIJALIISA, BRADER, GÜNTER, MULLINEAUX, PHIL, KANGASJÄRVI, JAAKKO
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-09-2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Antioxidants - metabolism; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Biological and medical sciences; Cell Death; Chlorophyll - metabolism; chloroplast; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; genetics; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - pharmacology; leaf organogenesis; Mutation; Non agrochemicals pollutants; oxidative stress; Ozone - metabolism; Ozone - pharmacology; Phenotype; Phytopathology. Animal pests. Plant and forest protection; Plant Growth Regulators - metabolism; Plant Leaves - drug effects; Plant Stomata - drug effects; Plant Stomata - physiology; Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors; Pollution effects. Side effects of agrochemicals; reactive oxygen species; reticulata; stomata; Superoxides - metabolism; Superoxides - pharmacology; Oxidative stress; reticulata; Ozone; Stomata; leaf organogenesis; Plant leaf; Arabidopsis thaliana; genetics; Cruciferae; Dicotyledones; Angiospermae; reactive oxygen species; Spermatophyta; Mutation; Chloroplast
• ISSN: 0140-7791; 1365-3040; 1365-3040
• DOI: 10.1111/j.1365-3040.2008.01837.x

Genetically tractable model plants offer the possibility of defining the plant O₃ response at the molecular level. To this end, we have isolated a collection of ozone (O₃)-sensitive mutants of Arabidopsis thaliana. Mutant phenotypes and genetics were characterized. Additionally, parameters associated with O₃ sensitivity were analysed, including stomatal conductance, sensitivity to and accumulation of reactive oxygen species, antioxidants, stress gene-expression and the accumulation of stress hormones. Each mutant has a unique phenotypic profile, with O₃ sensitivity caused by a unique set of alterations in these systems. O₃ sensitivity in these mutants is not caused by gross deficiencies in the antioxidant pathways tested here. The rcd3 mutant exhibits misregulated stomata. All mutants exhibited changes in stress hormones consistent with the known hormonal roles in defence and cell death regulation. One mutant, dubbed re-8, is an allele of the classic leaf development mutant reticulata and exhibits phenotypes dependent on light conditions. This study shows that O₃ sensitivity can be determined by deficiencies in multiple interacting plant systems and provides genetic evidence linking these systems.