Variation of mucin adhesion, cell surface characteristics, and molecular mechanisms among Lactobacillus plantarum isolated from different habitats

• Published in: Applied microbiology and biotechnology Vol. 101; no. 20; pp. 7663 - 7674
• Main Authors: Buntin, Nirunya, de Vos, Willem M., Hongpattarakere, Tipparat
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-10-2017; Springer; Springer Nature B.V
• Subjects: Adhesins, Bacterial - analysis; Adhesins, Bacterial - genetics; Adhesion; Adhesives; Agglutination; Animals; Applied Microbial and Cell Physiology; Bacterial Adhesion; Biomedical and Life Sciences; Biotechnology; Cell adhesion; Cell surface; Cell walls; Competitive adhesion; Flagellin; Food; Food Microbiology; Food plants; Gastrointestinal Tract - microbiology; Gene-trait matching; Genomes; Genotype; Guanidine hydrochloride; Humans; Hydrophobicity; Infant; Infants; Intestine; Lactobacillus; Lactobacillus fliC; Lactobacillus plantarum; Lactobacillus plantarum - genetics; Lactobacillus plantarum - isolation & purification; Lactobacillus plantarum - physiology; Life Sciences; Lithium chloride; Mannose; Mannose-specific adhesion; Microbial Genetics and Genomics; Microbiology; Molecular modelling; Mucin; Mucin-adhesion mechanism; Mucins; Mucins - metabolism; Mucus-binding protein; Observations; Penaeidae - microbiology; Physiological aspects; Pretreatment; Proteins; Surface Properties; Yeast; Yeasts; Gene-trait matching; Competitive adhesion; Mannose-specific adhesion; Mucin-adhesion mechanism; Mucus-binding protein; Lactobacillus; Lactobacillus fliC
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-017-8482-3

The adhesion ability to mucin varied greatly among 18
Lactobacillus plantarum
isolates depending on their isolation habitats. Such ability remained at high level even though they were sequentially exposed to the gastrointestinal (GI) stresses. The majority of
L. plantarum
isolated from shrimp intestine and about half of food isolates exhibited adhesion ability (51.06–55.04%) about the same as the well-known adhesive
L. plantarum
299v. Interestingly, five infant isolates of CIF17A2, CIF17A4, CIF17A5, CIF17AN2, and CIF17AN8 exhibited extremely high adhesion ranging from 62.69 to 72.06%. Such highly adhesive property correlating to distinctively high cell surface hydrophobicity was significantly weaken after pretreatment with LiCl and guanidine-HCl confirming the entailment of protein moiety. Regarding the draft genome information, all molecular structures of major cell wall-anchored proteins involved in the adhesion based on
L. plantarum
WCSF1, including lp_0964, lp_1643, lp_3114, lp_2486, lp_3127, and lp_3059 orthologues were detected in all isolates. Exceptionally, the gene-trait matching between yeast agglutination assay and the relevant mannose-specific adhesin (lp_1229) encoding gene confirmed the Msa absence in five infant isolates expressed distinctively high adhesion. Interestingly, the predicted flagellin encoding genes (
fliC
) firstly revealed in lp_1643, lp_2486, and lp_3114 orthologues may potentially contribute to such highly adhesive property of these isolates.