Construction of random small-insert genomic libraries highly enriched for simple sequence repeats

We present an efficient method for construction of random small-insert genomic libraries enriched for (CA) sub(n) repeats. The method involves fragmentation of DNA by sonication and ligation of an adaptor to the fragments followed by PCR amplification and enrichment for (CA) sub(n) repeats by hybrid...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 21; no. 16; pp. 3911 - 3912
Main Authors:	Karagyozov, L, Kalcheva, I.D, Chapman, V.M
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 11-08-1993
Subjects:	Animals Base Sequence biochemical techniques Biological and medical sciences Cloning, Molecular dinucleotide repeat sequences Diverse techniques DNA DNA libraries Fundamental and applied biological sciences. Psychology Genomic Library Humans Mice Molecular and cellular biology Molecular Sequence Data nucleic acid hybridization Polymerase Chain Reaction repetitive sequences Repetitive Sequences, Nucleic Acid Sonication Enrichment Polymerase chain reaction Genetic marker Repeated sequence DNA Gene library Method Molecular cloning
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Summary:	We present an efficient method for construction of random small-insert genomic libraries enriched for (CA) sub(n) repeats. The method involves fragmentation of DNA by sonication and ligation of an adaptor to the fragments followed by PCR amplification and enrichment for (CA) sub(n) repeats by hybridization to (GT) sub(15) Oligonucleotides bound to a nylon membrane.
Bibliography:	On leave from the Institute for Cell Biology and Morphology, Bulgarian Academy of Sciences ark:/67375/HXZ-GBNLH159-B ArticleID:21.16.3911 To whom correspondence should be addressed istex:FD2DE15A50C65C7F6752922320BC80B8D6B2D69A ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/21.16.3911