Tropheryma whipplei in the stool samples of children with acute diarrhea: a study from Tehran, Iran

• Published in: BMC infectious diseases Vol. 22; no. 1; p. 193
• Main Authors: Sayyahfar, Shirin, Latifian, Mina, Esmaeili, Parisa, Baseri, Neda, Bagheri Amiri, Fahimeh, Bakhshi, Bita, Esteghamati, Abdoulreza, Esmaeili, Saber
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27-02-2022; BioMed Central; BMC
• Subjects: Child; Child, Preschool; Development and progression; Diarrhea - epidemiology; Diarrhea in children; Diarrheas; Female; Gastrointestinal Disease; Gram-positive bacteria; Health aspects; Humans; Iran - epidemiology; Male; Pediatric research; Real-Time Polymerase Chain Reaction; Risk factors; Tropheryma - genetics; Tropheryma whipplei; Whipple Disease - diagnosis; Whipple's Disease; Iran; Tropheryma whipplei; Gastrointestinal Disease; Diarrheas; Whipple's Disease
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-022-07198-5

Recently, Tropheryma whipplei has been suggested as one of the causative agents of diarrhea among children worldwide. Limited data is available on the prevalence of T. whipplei among children with diarrhea in most countries such as Iran. This study was conducted to evaluate the prevalence of T. whipplei in children with acute diarrhea in Iran.
In this study, the stool samples were collected from 130 children under 10 years old with acute diarrhea from children's hospitals in Tehran city. Genomic DNA was extracted from stool samples and was tested for the presence of DNA of T. whipplei using the SYBR Green Real-time PCR method. Positive T. whipplei samples were finally confirmed by PCR Product sequencing.
The mean age of participants was 32.5 months, and 54.6% of children were female. Using the SYBR Green Real-time PCR, 9.23% (12/130) of samples were positive for T. whipplei, which were confirmed by sequencing. 66.67% of positive cases were males. The duration of diarrhea in infected children with T. whipplei (83.3%) was significantly longer (OR: 5.93, 95% CI 1.24-28.22) compared to children with negative results (45.8%). Other demographic factors and clinical signs had not a statistically significant relationship with T. whipplei infection.
In this study, T. whipplei was detected in stool samples of children with acute diarrhea. The results indicated that T. whipplei could be associated with childhood diarrhea in Iran. The health care system and physicians should be aware of the presence of T. whipplei infection in Iran, especially in childhood diarrhea.