Increased production of the antibiotic aurodox (X-5108) by aurodox-resistant mutants

• Published in: Journal of antibiotics Vol. 31; no. 7; p. 662
• Main Authors: Unowsky, J, Hoppe, D C
• Format: Journal Article
• Language: English
• Published: Japan 01-01-1978
• Subjects: Aurodox - biosynthesis; Aurodox - pharmacology; Drug Resistance, Microbial; Fermentation; Mutagens - pharmacology; Streptomyces - drug effects; Streptomyces - genetics; Streptomyces - metabolism
• ISSN: 0021-8820
• DOI: 10.7164/antibiotics.31.662

Conventional strain and media improvement techniques were of limited success in increasing yields of the antibiotic aurodox (X-5108) above 0.5 g/liter. Higher yields were obtained by reversion of a zero producer followed by the selection of mutants resistant to aurodox. Resistant strains of Streptomyces goldiniensis ATCC 21386 able to grow on 2 g/liter of aurodox produced greater than 2.5 g/liter of antibiotic. The rate of yield increase leveled off as the strains became resistant to greater than 2 g/liter of aurodox. These strains, in contrast to those sensitive to aurodox, gave a positive response to conventional mutagenic methods for further yield increases.