circ-TFRC downregulation suppresses ovarian cancer progression via miR-615-3p/IGF2 axis regulation

• Published in: Cancer cell international Vol. 24; no. 1; p. 152
• Main Authors: Yan, Zhongxin, Duan, Changling, Li, Xi, Wang, Hao, Li, Shanji, Zhou, Xuexin, Miao, Yi
• Format: Journal Article
• Language: English
• Published: England BioMed Central 27-04-2024; BMC
• Subjects: Bioinformatics; Biotechnology; Brain cancer; Cancer therapies; Cell adhesion & migration; Cell growth; Cell migration; Cell proliferation; circ-TFRC; Circular RNA; Down-regulation; IGF2; Insulin-like growth factor II; Malignancy; Medical prognosis; Metastases; Metastasis; MicroRNAs; miR-615-3p; Ovarian cancer; Tumorigenesis; United States > US; China; circ-TFRC; IGF2; miR-615-3p; Ovarian cancer
• ISSN: 1475-2867; 1475-2867
• DOI: 10.1186/s12935-024-03287-4

Ovarian cancer (OC) is a malignancy among female globally. Circular RNAs (circRNAs) are a family of circular endogenous RNAs generated from selective splicing, which take part in many traits. Former investigation suggested that circ-TFRC was abnormally expressed in breast cancer (BC). Further, the role of circ-TFRC to the progress of OC remains unclear. So, the aim of this study was to reveal the regulatory mechanism of circ-TFRC.
Our team made the luciferase reporter assay to validate circ-TFRC downstream target. Transwell migration assay, 5-ethynyl-20-deoxyuridine, and cell counting kit-8 were applied to investigate both proliferation and migration. In vivo tumorigenesis and metastasis assays were performed to investigate the circ-TFRC role in OC.
The outputs elucidated that circ-TFRC expression incremented in OC cells and tissues. circ-TFRC downregulation inhibited OC cell proliferation as well as migration in in vivo and in vitro experiments. The luciferase results validated that miR-615-3p and IGF2 were circ-TFRC downstream targets. IGF2 overexpression or miR-615-3p inhibition reversed OC cell migration after circ-TFRC silencing. Also, IGF2 overexpression reversed OC cell migration and proliferation post miR-615-3p upregulation.
Results demonstrate that circ-TFRC downregulation inhibits OC progression and metastasis via IGF2 expression regulation and miR-615-3psponging.