Determining the cytotoxicity of the Minimum Inhibitory Concentration (MIC) of silver and zinc oxide nanoparticles in ESBL and carbapenemase producing Proteus mirabilis isolated from clinical samples in Shiraz, Southwest Iran

• Published in: BMC research notes Vol. 17; no. 1; pp. 40 - 6
• Main Authors: Kakian, Farshad, Mirzaei, Esmaeil, Moattari, Afagh, Takallu, Sara, Bazargani, Abdollah
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 29-01-2024; BioMed Central; BMC
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacterial Proteins; Beta lactamases; Biocompatibility; Carbapenemase; Cell culture; Cell Culture techniques; Cells; Control; Cytotoxicity; Diffraction; Drug resistance; Drug resistance in microorganisms; Electron microscopy; Enzymes; Health aspects; Humans; Identification and classification; Imipenem; Infection; Iran; Medical colleges; Medical research; Medicine, Experimental; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Minimum inhibitory concentration; Morphology; Nanoparticles; Polymerase chain reaction; Prevention; Properties; Proteus mirabilis; Proteus mirabilis, Drug Resistance; Research Note; Silver; Silver - chemistry; Silver - pharmacology; Statistical analysis; X-ray diffraction; X-rays; Zinc oxide; Zinc Oxide - chemistry; Zinc Oxide - pharmacology; β Lactamase; Iran; Germany; Silver; Zinc oxide; Proteus mirabilis, Drug Resistance; Cell Culture techniques
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-023-06402-2

Proteus mirabilis is related to serious infections. The present study was designed to investigate the minimum inhibitory concentration (MIC) of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) and cytotoxicity among P. mirabilis isolates recovered from clinical samples in Shiraz.
A total of 100 P. mirabilis isolates were screened by biochemical tests and polymerase chain reaction (PCR). Also, 25 (25%) and 7 (7%) isolates were positive for extended-spectrum beta-lactamase (ESBLs) and carbapenemase, respectively. Synthesized nanoparticles were characterized by UV-vis spectrum, X-ray diffraction (XRD), and electron microscopy. The average size of AgNPs and ZnONPs in the present study is 48 and < 70 nm, respectively. The MIC and the MBC of the ZnONPs were in the range of 31.25 µg/ml and 62.5 µg/mL, respectively. Also, for AgNPs, the MIC and the MBC were in the range of 7.8 µg/mL and 15.6 µg/mL, respectively. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in a primary culture of fibroblast L929 cells for this MIC indicated biocompatibility and low cytotoxicity of Ag NPs and for ZnONPs indicated significant cytotoxicity. Also, a MIC of AgNPs can be used as a therapeutic concentration without the effect of cytotoxicity in human cells.