Comparative transcriptome analysis of root, stem, and leaf tissues of Entada phaseoloides reveals potential genes involved in triterpenoid saponin biosynthesis

• Published in: BMC genomics Vol. 21; no. 1; p. 639
• Main Authors: Liao, Weifang, Mei, Zhinan, Miao, Lihong, Liu, Pulin, Gao, Ruijie
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15-09-2020; BioMed Central; BMC
• Subjects: Analysis; Bioactive compounds; Biosynthesis; Cytochrome P450; Cytochromes P450; Entada phaseoloides; Enzymes; Fabaceae - genetics; Fabaceae - metabolism; Flavonoids; Gene expression; Genes; Genes, Plant; Genetic aspects; Genetic engineering; Genomics; Glycosyltransferase; Herbal medicine; Hydrocarbons; Inflammation; Kinases; Leaves; Legumes; Medicinal plants; Metabolism; Metabolites; Methods; Moisture content; Photosynthesis; Physiological aspects; Plant Components, Aerial - genetics; Plant Components, Aerial - metabolism; Plant Roots - genetics; Plant Roots - metabolism; Plant tissues; Saponins; Saponins - biosynthesis; Saponins - genetics; Secondary Metabolism; Secondary metabolites; Signal transduction; Stems; Transcription (Genetics); Transcriptome; Triterpenoid saponins; Uridine; China; Secondary metabolites; Entada phaseoloides; Triterpenoid saponins; Transcriptome
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-020-07056-1

Entada phaseoloides (L.) Merr. is an important traditional medicinal plant. The stem of Entada phaseoloides is popularly used as traditional medicine because of its significance in dispelling wind and dampness and remarkable anti-inflammatory activities. Triterpenoid saponins are the major bioactive compounds of Entada phaseoloides. However, genomic or transcriptomic technologies have not been used to study the triterpenoid saponin biosynthetic pathway in this plant.
We performed comparative transcriptome analysis of the root, stem, and leaf tissues of Entada phaseoloides with three independent biological replicates and obtained a total of 53.26 Gb clean data and 116,910 unigenes, with an average N50 length of 1218 bp. Putative functions could be annotated to 42,191 unigenes (36.1%) based on BLASTx searches against the Non-redundant, Uniprot, KEGG, Pfam, GO, KEGG and COG databases. Most of the unigenes related to triterpenoid saponin backbone biosynthesis were specifically upregulated in the stem. A total of 26 cytochrome P450 and 17 uridine diphosphate glycosyltransferase candidate genes related to triterpenoid saponin biosynthesis were identified. The differential expressions of selected genes were further verified by qPT-PCR.
The dataset reported here will facilitate the research about the functional genomics of triterpenoid saponin biosynthesis and genetic engineering of Entada phaseoloides.