Family Trees Representing the Finitely Proliferative Nature of Cultured Rat Liver Cells

• Published in: Cell Structure and Function Vol. 8; no. 3; pp. 293 - 301
• Main Authors: Matsumura, Toshiharu, Masuda, Kiyofumi, Murakami, Yoshinori, Konishi, Ryuji
• Format: Journal Article
• Language: English
• Published: Kyoto Japan Society for Cell Biology 01-01-1983; Japan Science and Technology Agency
• Subjects: Animals; Biological and medical sciences; Cell cycle, cell proliferation; Cell Line; Cell physiology; Cell Survival; Cells, Cultured; Clone Cells; Fundamental and applied biological sciences. Psychology; Liver - cytology; Molecular and cellular biology; Rats; Rats, Inbred Strains; Cell proliferation; Cell culture; Vertebrata; Mammalia; Genealogy; Rat; Liver; Rodentia; Cinematography; Epithelial cell
• ISSN: 0386-7196; 1347-3700
• DOI: 10.1247/csf.8.293

Family trees of rat liver epithelial-like cells in primary culture were obtained by cinemicrographic analysis. With few exceptions the proliferative profiles reconstituted from these family trees showed limited proliferation. This limitation was not due to post-confluence inhibition of proliferation or to nutritional deprivation during time lapse cinemicrography, but to the finite potential of clonal proliferation. In these family trees a cell entered mitosis, incomplete division, death, or a long interphase with no detectable termination. These family trees successfully related the life phases of the individual cells that compose the population to the limited nature of proli-ferative potential as observed in clonal cell populations.